Anti-Human I-TAC Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC, E |
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Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Preparation | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hI-TAC. Anti-Human I-TAC specific antibody was purified by affinity chromatography employing immobilized hI-TAC matrix. |
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WesternBlot | To detect hI-TAC by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hI-TAC is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. |
Sandwich | To detect hI-TAC by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human I-TAC (60-197BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hI-TAC. |
Immunohistochemistry | This antibody stained formalin-fixed paraffin-embedded sections of human renal tumor with parenchyma tissue. The recommended concentration is 0.125 µg/mL- 0.25 µg/mL with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved without antigen retrieval. Optimal concentrations and conditions may vary. |
Neutralization | To yield one-half maximal inhibition [ND50] of the biological activity of hI-TAC (100 ng/ml), a concentration of 7.0-8.0 µg/ml of this antibody is required. |
Formulation | A sterile filtered antibody solution was lyophilized from PBS, pH 7.2. |
Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
Storage | -20°C |
Precautions | Anti-Human I-TAC Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
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