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> home > Products > Primary Antibodies > Antibody Collections > Antibody Pairs > Anti-Human sTNF Receptor Type II Antibody
Anti-Human sTNF Receptor Type II Antibody
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Application 
| WB, IHC, E |
|---|---|
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Preparation | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hsTNF-receptor II. Anti-Human sTNF-receptor II specific antibody was purified by affinity chromatography employing immobilized hsTNF-receptor II matrix. |
|---|---|
| WesternBlot | To detect hsTNF-receptor II by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsTNF-receptor II is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. |
| Sandwich | To detect hsTNF-receptor II by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human sTNF-receptor II (60-328BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsTNF-receptor II. |
| Immunohistochemistry | This antibody stained PC-3 and CaCo-2 cells. The primary antibody was incubated at 3.0 mg/ml overnight at 4°C followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Information is courtesy of the Cell Profiling group, SciLifeLab Stockholm. |
| Neutralization | To yield one-half maximal inhibition [ND50] of the biological activity of Human sTNF Receptor-II (0.5 μg/ml) a concentration of 15.0 - 20.0 μg/ml of this antibody is required. |
| Formulation | A sterile filtered antibody solution was lyophilized from PBS, pH 7.2. |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Storage | -20°C |
| Precautions | Anti-Human sTNF Receptor Type II Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Research Areas
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Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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$ 275.00
$ 216.00
Cat# ABG10527-U100
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