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> home > Products > Primary Antibodies > Antibody Collections > Antibody Pairs > Anti-Human TIMP-1 Antibody
Anti-Human TIMP-1 Antibody
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Application 
| WB, IHC, E |
|---|---|
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Preparation | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hTIMP-1 (human Tissue Inhibitor of Metalloproteinases-1). Anti-Human TIMP-1 specific antibody was purified by affinity chromatography employing immobilized hTIMP-1 matrix. |
|---|---|
| WesternBlot | To detect hTIMP-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTIMP-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
| Sandwich | To detect hTIMP-1 by sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human TIMP-1 (60-319BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTIMP-1. |
| Immunohistochemistry | This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 0.1 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results were achieved with a proteinase K antigen retrieval. Optimal concentrations and conditions may vary. |
| Neutralization | To yield one-half maximal inhibition [ND50] of the biological activity of Human TIMP-1 (3.0 μg/ml), a concentration of 5.0 μg/ml of this antibody is required. |
| Formulation | A sterile filtered antibody solution was lyophilized from PBS, pH 7.2. |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Storage | -20°C |
| Precautions | Anti-Human TIMP-1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Research Areas
Citations (0)
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info@abcepta.com, and receive a free "I Love Antibodies" mug.
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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$ 275.00
$ 216.00
Cat# ABG10541-U100
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