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Anti-VEGF Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | Q00731 |
| Host | Rabbit |
| Reactivity | Mouse, Rat |
| Clonality | Polyclonal |
| Format | Lyophilized |
| Description | Rabbit IgG polyclonal antibody for Vascular endothelial growth factor A(VEGFA) detection. Tested with WB in Mouse;Rat. |
| Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
| Gene ID | 22339 |
|---|---|
| Other Names | Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, Vegfa, Vegf |
| Calculated MW | 25283 MW KDa |
| Application Details | Western blot, 0.1-0.5 µg/ml, Rat, Mouse |
| Subcellular Localization | Isoform VEGF-1: Secreted. |
| Tissue Specificity | In developing embryos, expressed mainly in the choroid plexus, paraventricular neuroepithelium, placenta and kidney glomeruli. Also found in bronchial epithelium, adrenal gland and in seminiferous tubules of testis. High expression of VEGF continues in kidney glomeruli and choroid plexus in adults. |
| Protein Name | Vascular endothelial growth factor A |
| Contents | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
| Immunogen | E.coli-derived mouse VEGF recombinant protein (Position: A27-R214). Mouse VEGF shares 89.4% and 98.4% amino acid (aa) sequence identity with human and rat VEGF, respectively. |
| Purification | Immunogen affinity purified. |
| Cross Reactivity | No cross reactivity with other proteins. |
| Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
| Name | Vegfa |
|---|---|
| Synonyms | Vegf |
| Function | [N-VEGF]: Participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A (PubMed:35455969). Involved in protecting cells from hypoxia- mediated cell death (PubMed:35455969). |
| Cellular Location | [N-VEGF]: Cytoplasm {ECO:0000250|UniProtKB:P15692}. Nucleus {ECO:0000250|UniProtKB:P15692} Note=Cytoplasmic in normoxic conditions and localizes to the nucleus under hypoxic conditions. {ECO:0000250|UniProtKB:P15692} [Isoform L-VEGF-2]: Endoplasmic reticulum {ECO:0000250|UniProtKB:P15692}. Golgi apparatus {ECO:0000250|UniProtKB:P15692}. Secreted, extracellular space, extracellular matrix {ECO:0000250|UniProtKB:P15692} [Isoform VEGF-2]: Secreted. |
| Tissue Location | In developing embryos, expressed mainly in the choroid plexus, paraventricular neuroepithelium, placenta and kidney glomeruli. Also found in bronchial epithelium, adrenal gland and in seminiferous tubules of testis. High expression continues in kidney glomeruli and choroid plexus in adults |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
VEGF, a homodimeric glycoprotein of relative molecular mass 45,000, is the only mitogen that specifically acts on endothelial cells. It may be a major regulator of tumor angiogenesis in vivo. It is, however, structurally related to platelet-derived growth factor. VEGF shares homology with the PDGF A chain and B chain, including conservation of all 8 cysteines found in PDGFA and PDGFB. VEGF gene contains 8 exons. And VEGF induces remodeling and enhances TH2-mediated sensitization and inflammation in the lung. This gene can also regulate haematopoietic stem cell survival by an internal autocrine loop mechanism. What’s more, it also stimulates neurogenesis in vitro and in vivo.
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