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Anti-BIN1 Antibody (Monoclonal, 99D)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-F, ICC |
|---|---|
| Primary Accession | O08839 |
| Host | Mouse |
| Isotype | Mouse IgG2b |
| Reactivity | Human, Mouse, Rat |
| Clonality | Monoclonal |
| Format | Lyophilized |
| Description | Mouse IgG monoclonal antibody for BIN1, bridging integrator 1 (BIN1) detection. Tested with WB, IHC-F, ICC in Human;mouse;rat. No cross reactivity with other proteins. |
| Reconstitution | Add 1ml of PBS buffer will yield a concentration of 100ug/ml. |
| Gene ID | 117028 |
|---|---|
| Other Names | Myc box-dependent-interacting protein 1, Amphiphysin II, Amphiphysin-like protein, Bridging integrator 1, Bin1, Amph2, Amphl |
| Calculated MW | 64533 MW KDa |
| Application Details | Immunocytochemistry , 1 µg/ml, Human, mouse, rat, - Immunohistochemistry(Frozen Section), 0.5 µg/ml, Human, mouse, rat, - Western blot, 0.25 µg/ml, Human, mouse, rat |
| Subcellular Localization | Cytoplasm . Nucleus . |
| Tissue Specificity | Isoform AMPH2-1 is expressed in brain, concentrated at nerve terminals. Isoform AMPH2-2 is widely expressed. |
| Protein Name | Myc box-dependent-interacting protein 1 |
| Contents | Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative. |
| Clone Names | 99D |
| Immunogen | Recombinant polypeptide containing amino acids 189-398 of human Bin1. |
| Purification | Ascites |
| Cross Reactivity | No cross reactivity with other proteins |
| Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
| Sequence Similarities | Contains 1 BAR domain. |
| Name | Bin1 |
|---|---|
| Synonyms | Amph2, Amphl |
| Function | Is a key player in the control of plasma membrane curvature, and membrane shaping and remodeling. Required in muscle cells for the formation of T-tubules, tubular invaginations of the plasma membrane that function in depolarization-contraction coupling. Required in muscle cells for the formation of T-tubules, tubular invaginations of the plasma membrane that function in depolarization-contraction coupling (By similarity). Is a negative regulator of endocytosis (PubMed:27760323, PubMed:9736607). Is also involved in the regulation of intracellular vesicles sorting, modulation of BACE1 trafficking and the control of amyloid-beta production (By similarity). In neuronal circuits, endocytosis regulation may influence the internalization of PHF-tau aggregates (PubMed:27760323). May be involved in the regulation of MYC activity and the control cell proliferation (By similarity). |
| Cellular Location | Nucleus {ECO:0000250|UniProtKB:O08539}. Cytoplasm {ECO:0000250|UniProtKB:O08539}. Endosome {ECO:0000250|UniProtKB:O08539}. Cell membrane, sarcolemma, T-tubule |
| Tissue Location | Highly expressed in the brain and muscle. Isoform AMPH2-1 is expressed only in the brain where it is concentrated in axon initial segments and nodes of Ranvier. Isoform AMPH2-2 is widely expressed. |
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Provided below are standard protocols that you may find useful for product applications.
Background
BIN1(AMPH2) is a novel human gene product with features of a tumor suppressor protein. BIN1 gene to chromosome 2q14. Loss of BIN1 expression appears to be a frequent aberration in human hepatocellular carcinomas . mutations in BIN1 cause centronuclear myopathy by interfering with remodeling of T tubules and/or endocytic membranes, and that the functional interaction between BIN1 and DNM2 is necessary for normal muscle function and positioning of nuclei.
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