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Anti-Calbindin-D Antibody (Monoclonal, CB-955)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P |
|---|---|
| Primary Accession | P07171 |
| Host | Mouse |
| Isotype | Mouse IgG1 |
| Reactivity | Human, Mouse, Rat |
| Clonality | Monoclonal |
| Format | Lyophilized |
| Description | Mouse IgG monoclonal antibody for Calbindin-D, calbindin 1, 28kDa (CALB1) detection. Tested with WB, IHC-P in Human;mouse;rat;bovine. No cross reactivity with other proteins. |
| Reconstitution | Add 1ml of PBS buffer will yield a concentration of 100ug/ml. |
| Gene ID | 83839 |
|---|---|
| Other Names | Calbindin, Calbindin D28, D-28K, Spot 35 protein, Vitamin D-dependent calcium-binding protein, avian-type, Calb1 |
| Calculated MW | 29994 MW KDa |
| Application Details | Immunohistochemistry(Paraffin-embedded Section), 1-2 µg/ml, Human, bovine, mouse, rat, By Heat Western blot, 0.5-1 µg/ml, Human, bovine, mouse, rat |
| Protein Name | Calbindin |
| Contents | Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative. |
| Clone Names | CB-955 |
| Immunogen | Bovine kidney calbindin-D |
| Purification | Ascites |
| Cross Reactivity | No cross reactivity with other proteins |
| Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
| Sequence Similarities | Belongs to the calbindin family. |
| Name | Calb1 |
|---|---|
| Function | Buffers cytosolic calcium. May stimulate a membrane Ca(2+)- ATPase and a 3',5'-cyclic nucleotide phosphodiesterase. |
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Provided below are standard protocols that you may find useful for product applications.
Background
The human calbindin D28k(CALB1) is a calcium-binding protein belonging to the troponin C superfamily. It was originally described as a 27-kD protein induced by vitamin D in the duodenum of the chick. Parmentier et al.(1991) mapped the CALB1 gene to 8q21.3-q22.1 by in situ hybridization.
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