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Anti-CDK4 Antibody (Monoclonal, DCS-31)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, ICC |
|---|---|
| Primary Accession | P35462 |
| Host | Mouse |
| Isotype | Mouse IgG2a |
| Reactivity | Human, Mouse, Rat |
| Clonality | Monoclonal |
| Format | Lyophilized |
| Description | Mouse IgG monoclonal antibody for CDK4, cyclin-dependent kinase 4 (CDK4) detection. Tested with WB, ICC in Human;mouse;rat. No cross reactivity with other proteins. |
| Reconstitution | Add 1ml of PBS buffer will yield a concentration of 100ug/ml. |
| Gene ID | 1814 |
|---|---|
| Other Names | D(3) dopamine receptor, Dopamine D3 receptor, DRD3 |
| Calculated MW | 44225 MW KDa |
| Application Details | Immunocytochemistry , 1 µg/ml, Human, mouse, rat, - Western blot, 0.5-1 µg/ml, Human, mouse, rat |
| Subcellular Localization | Cell membrane ; Multi-pass membrane protein . Both membrane-bound and scattered in the cytoplasm during basal conditions. Receptor stimulation results in the rapid internalization and sequestration of the receptors at the perinuclear area (5 and 15 minutes), followed by the dispersal of the receptors to the membrane (30 minutes). DRD3 and GRK4 co- localize in lipid rafts of renal proximal tubule cells. |
| Tissue Specificity | Brain. |
| Protein Name | Cyclin-dependent kinase 4 |
| Contents | Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative. |
| Clone Names | DCS-31 |
| Immunogen | Recombinant human Cdk4 protein. |
| Purification | Ascites |
| Cross Reactivity | No cross reactivity with other proteins |
| Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
| Sequence Similarities | Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. |
| Name | DRD3 (HGNC:3024) |
|---|---|
| Function | Dopamine receptor whose activity is mediated by G proteins which inhibit adenylyl cyclase. Promotes cell proliferation. |
| Cellular Location | Cell membrane; Multi-pass membrane protein. Note=Both membrane-bound and scattered in the cytoplasm during basal conditions Receptor stimulation results in the rapid internalization and sequestration of the receptors at the perinuclear area (5 and 15 minutes), followed by the dispersal of the receptors to the membrane (30 minutes). DRD3 and GRK4 co-localize in lipid rafts of renal proximal tubule cells |
| Tissue Location | Brain. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Cyclin-dependent kinase-4(CDK4) is a protein-serine kinase involved in the cell cycle. Human cell division is regulated primarily at the G1-to-S or the G2-to-M boundaries within the cell cycle. The complexes formed by CDK4 and the D-type cyclins are involved in the control of cell proliferation during the G1 phase. CDK4 is inhibited by p16, also known as cyclin-dependent kinase inhibitor-2. CDK4 is mapped to 12q14. CDK4 expression and activity are required for cytokine responsiveness in T cells.
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