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Anti-Apoptosis Inhibitor 5 Antibody

     
  • WB - Anti-Apoptosis Inhibitor 5 Antibody ABO10504
    Anti-Apoptosis inhibitor 5 antibody, ABO10504, Western blottingLane 1: Rat Cardiac Muscle Tissue LysateLane 2: Rat Brain Tissue LysateLane 3: Rat Testis Tissue LysateLane 4: Rat Placenta Tissue LysateLane 5: MCF-7 Cell LysateLane 6: HELA Cell LysateLane 7: CEM Cell LysateLane 8: SMMC Cell LysateLane 9: COLO320 Cell Lysate
    detail
  • IHC - Anti-Apoptosis Inhibitor 5 Antibody ABO10504
    Anti-Apoptosis inhibitor 5 antibody, ABO10504, IHC(P)IHC(P): Rat Brain Tissue
    detail
  • ICC - Anti-Apoptosis Inhibitor 5 Antibody ABO10504
    Anti-Apoptosis inhibitor 5 antibody, ABO10504, ICCICC: HELA Cell
    detail
  • IHC - Anti-Apoptosis Inhibitor 5 Antibody ABO10504
    Anti-Apoptosis inhibitor 5 antibody, ABO10504, IHC(F)IHC(F): Rat Cardiac Muscle Tissue
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, ICC
Primary Accession Q9BZZ5
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Apoptosis inhibitor 5(API5) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;Mouse;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 8539
Other Names Apoptosis inhibitor 5, API-5, Antiapoptosis clone 11 protein, AAC-11, Cell migration-inducing gene 8 protein, Fibroblast growth factor 2-interacting factor, FIF, Protein XAGL, API5
Calculated MW 59005 MW KDa
Application Details Immunocytochemistry , 0.5-1 µg/ml, Human, Mouse
Immunohistochemistry(Frozen Section), 0.5-1 µg/ml, Rat, Human, Mouse
Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5 µg/ml, Human, Rat, Mouse
Subcellular Localization Nucleus . Cytoplasm . Mainly nuclear. Can also be cytoplasmic.
Tissue Specificity Expressed in all tissues tested, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in heart, pancreas and placenta. Highly expressed in several cancers. Preferentially expressed in squamous cell carcinoma versus adenocarcinoma in non-small cell lung cancer. .
Protein Name Apoptosis inhibitor 5(API-5)
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human Apoptosis inhibitor 5(487-504aa KYSSNLGNFNYERSLQGK)(b), different from the related mouse sequence by one amino acid.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Sequence Similarities Belongs to the API5 family.
Protein Information
Name API5 (HGNC:594)
Function Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs.
Cellular Location Nucleus. Cytoplasm. Note=Mainly nuclear. Can also be cytoplasmic
Tissue Location Expressed in all tissues tested, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas Highest levels in heart, pancreas and placenta. Highly expressed in several cancers. Preferentially expressed in squamous cell carcinoma versus adenocarcinoma in non-small cell lung cancer
Research Areas
Citations (0)
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Background

Many growth factors and cytokines act as cellular survival factors by preventing programmed cell death(apoptosis). Apoptosis inhibitor 5(API5) is an antiapoptotic factor which may have a role in protein assembly. The API5 gene to chromosome 11p12 based on an alignment of the API5 sequence with the genomic sequence It is a critical determinant of E2F1-induced apoptosis, acting downstream of E2F to suppress E2F-dependent apoptosis without generally blocking E2F-dependent transcription.

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$ 280.00
Cat# ABO10504
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