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Anti-Actin Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P |
|---|---|
| Primary Accession | P63261 |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Clonality | Polyclonal |
| Format | Liquid |
| Description | Rabbit IgG polyclonal antibody for Actin, cytoplasmic 2(ACTG1) detection. Tested with WB, IHC-P in Human;Mouse;Rat. |
| Gene ID | 71 |
|---|---|
| Other Names | Actin, cytoplasmic 2, Gamma-actin, Actin, cytoplasmic 2, N-terminally processed, ACTG1, ACTG |
| Calculated MW | 41793 MW KDa |
| Application Details | Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Rat, Mouse, By Heat Western blot, 0.1-0.5 µg/ml, Human, Rat, Mouse |
| Subcellular Localization | Cytoplasm, cytoskeleton. |
| Protein Name | Actin, cytoplasmic 2 |
| Contents | Each vial contains 50% glycerol, 0.9mg NaCl, 0.2mg Na2HPO4. Carrier free (No BSA) form available in stock. If you want this antibody carrier free please specify Carrier Free" or "No BSA" in your order note. " |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human Actin(365-375aa SGPSIVHRKCF), identical to the related mouse and rat sequences. |
| Purification | Immunogen affinity purified. |
| Cross Reactivity | No cross reactivity with other proteins |
| Storage | At -20˚C for one year, at 4˚C for one month. Avoid repeated freezing and thawing. |
| Sequence Similarities | Belongs to the actin family. |
| Name | ACTG1 |
|---|---|
| Synonyms | ACTG |
| Function | Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. |
| Cellular Location | Cytoplasm, cytoskeleton |
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Provided below are standard protocols that you may find useful for product applications.
Background
Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures in the cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three alpha-actin, one beta-actin, two gamma-actin. Because the amino acid sequence of the C-terminal is the same for almost all actins, this antibody has been raised using a synthetic peptide corresponding to the C-terminal 11 residues.
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