Anti-MGMT Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB |
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Primary Accession | P16455 |
Host | Rabbit |
Reactivity | Human, Mouse, Rat |
Clonality | Polyclonal |
Format | Lyophilized |
Description | Rabbit IgG polyclonal antibody for Methylated-DNA--protein-cysteine methyltransferase(MGMT) detection. Tested with WB in Human;Mouse;Rat. |
Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Gene ID | 4255 |
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Other Names | Methylated-DNA--protein-cysteine methyltransferase, 2.1.1.63, 6-O-methylguanine-DNA methyltransferase, MGMT, O-6-methylguanine-DNA-alkyltransferase, MGMT |
Calculated MW | 21646 MW KDa |
Application Details | Western blot, 0.1-0.5 µg/ml, Human, Rat, Mouse |
Subcellular Localization | Nucleus. |
Protein Name | Methylated-DNA--protein-cysteine methyltransferase |
Contents | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human MGMT(87-101aa PVFQQESFTRQVLWK), different from the related rat and mouse sequences by one amino acid. |
Purification | Immunogen affinity purified. |
Cross Reactivity | No cross reactivity with other proteins |
Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
Name | MGMT |
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Function | Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. |
Cellular Location | Nucleus. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Methylated-DNA-protein-cysteine methytransferase(MGMT) is an enzyme that in humans is encoded by the MGMT gene. O(6)-alkylguanine is the major mutagenic and carcinogenic lesion in DNA induced by simple alkylating mutagens. MGMT gene is mapped to chromosome 10. Rydberg et al.(1990) concluded that the methyltransferase gene spans at least 15 kb. Gardner et al.(1991) refined the localization of the gene to 10q24.33-qter. Methylation of the gene's promoter plays a significant role in carcinogenesis.
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