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Anti-Cytoglobin Antibody

     
  • WB - Anti-Cytoglobin Antibody ABO10972
    Anti-Cytoglobin antibody, ABO10972, Western blottingAll lanes: Anti Cytoglobin (ABO10972) at 0.5ug/mlLane 1: Rat Brain Tissue Lysate at 50ugLane 2: Rat Small Intestine Tissue Lysate at 50ugLane 3: Rat Liver Tissue Lysate at 50ugLane 4: Rat Kidney Tissue Lysate at 50ugLane 5: SGC Whole Cell Lysate at 40ugLane 6: COLO320 Whole Cell Lysate at 40ugLane 7: SMMC Whole Cell Lysate at 40ugLane 8: PANC Whole Cell Lysate at 40ugLane 9: HELA Whole Cell Lysate at 40ugPredicted bind size: 21KDObserved bind size: 21KD
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q8WWM9
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Cytoglobin(CYGB) detection. Tested with WB in Human;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 114757
Other Names Cytoglobin, Histoglobin, HGb, Stellate cell activation-associated protein, CYGB, STAP
Calculated MW 21405 MW KDa
Application Details Western blot, 0.1-0.5 µg/ml, Human, Rat, Mouse
Subcellular Localization Cytoplasm .
Tissue Specificity Ubiquitously expressed. Highest expression in heart, stomach, bladder and small intestine. .
Protein Name Cytoglobin
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Immunogen A synthetic peptide corresponding to a sequence at the N-terminus of human Cytoglobin(50-68aa FVNFPSAKQYFSQFKHMED), identical to the related rat sequence, different from the related mouse sequence by one amino acid.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Protein Information
Name CYGB
Synonyms STAP
Function May have a protective function during conditions of oxidative stress. May be involved in intracellular oxygen storage or transfer.
Cellular Location Cytoplasm.
Tissue Location Ubiquitously expressed. Highest expression in heart, stomach, bladder and small intestine
Research Areas
Citations (0)
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Background

Cytoglobin(CYGB), also called HGB or STAP, is a ubiquitously expressed hexacoordinate hemoglobin that may facilitate diffusion of oxygen through tissues, scavenge nitric oxide or other reactive oxygen species, or serve a protective function during oxidative stress. The cytoglobin gene is mapped on 17q25.1. The CYGB gene contains 4 exons and spans about 9 kb. Cytoglobin has many elements common to vertebrate globins, including invariant histidine residues, and the amino acids that form the heme pocket share similarity with pentacoordinate myoglobin. In contrast to the high oxygen affinities displayed by most hexacoordinate hemoglobins, the characteristics of CYGB indicate that it can facilitate oxygen transport. Because the oxygen affinity of CYGB is more similar to myoglobin than to neuroglobin, and the oxy form of CYGB resists autooxidation, CYGB is proposed to represent a tissue oxygen reservoir by Sawai et al.

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$ 240.00
Cat# ABO10972
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