Anti-P-Selectin Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P |
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Primary Accession | P16109 |
Host | Rabbit |
Reactivity | Human |
Clonality | Polyclonal |
Format | Lyophilized |
Description | Rabbit IgG polyclonal antibody for P-selectin(SELP) detection. Tested with WB, IHC-P in Human. |
Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Gene ID | 6403 |
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Other Names | P-selectin, CD62 antigen-like family member P, Granule membrane protein 140, GMP-140, Leukocyte-endothelial cell adhesion molecule 3, LECAM3, Platelet activation dependent granule-external membrane protein, PADGEM, CD62P, SELP, GMRP, GRMP |
Calculated MW | 90834 MW KDa |
Application Details | Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, By Heat Western blot, 0.1-0.5 µg/ml, Human |
Subcellular Localization | Membrane; Single-pass type I membrane protein. |
Tissue Specificity | Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface. |
Protein Name | P-selectin |
Contents | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human CD62P(49-66aa KAYSWNISRKYCQNRYTD). |
Purification | Immunogen affinity purified. |
Cross Reactivity | No cross reactivity with other proteins |
Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
Sequence Similarities | Belongs to the selectin/LECAM family. |
Name | SELP |
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Synonyms | GMRP, GRMP |
Function | Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with SELPLG. |
Cellular Location | Cell membrane; Single-pass type I membrane protein |
Tissue Location | Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface |
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Background
P-Selectin, also called CD62, GRMP, GMP140 is a 140-kD adhesion molecule, expressed at the surface of activated cells, that mediates the interaction of activated endothelial cells or platelets with leukocytes. The P-Selectin gene is mapped on 1q24.2. P-selectin plays an essential role in the initial recruitment of leukocytes(white blood cells) to the site of injury during inflammation. P-selectin is also very important in the recruitment and aggregation of platelets at areas of vascular injury. In quiescent platelet, P-selectin is located on the inner wall of alpha-granules. Sorting nexin-17 interacts with the cytosolic domain of P-selectin and suggested that SNX17 may function in the intracellular trafficking of P-selectin. P-selectin expression was found to be significantly and positively correlated with carotid artery stiffness and wall thickness, and the percentage of P-selectin-positive platelets was significantly higher in patients with carotid plaque.
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