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Anti-PERK Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, ICC |
|---|---|
| Primary Accession | Q9NZJ5 |
| Host | Rabbit |
| Reactivity | Human |
| Clonality | Polyclonal |
| Format | Lyophilized |
| Description | Rabbit IgG polyclonal antibody for Eukaryotic translation initiation factor 2-alpha kinase 3(EIF2AK3) detection. Tested with WB, ICC in Human. |
| Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
| Gene ID | 9451 |
|---|---|
| Other Names | Eukaryotic translation initiation factor 2-alpha kinase 3, 2.7.11.1, PRKR-like endoplasmic reticulum kinase, Pancreatic eIF2-alpha kinase, HsPEK, EIF2AK3, PEK, PERK |
| Calculated MW | 125216 MW KDa |
| Application Details | Western blot, 0.1-0.5 µg/ml, Human, - Immunocytochemistry , 0.5-1 µg/ml, Human |
| Subcellular Localization | Endoplasmic reticulum membrane; Single-pass type I membrane protein. |
| Tissue Specificity | Ubiquitous. A high level expression is seen in secretory tissues. |
| Protein Name | Eukaryotic translation initiation factor 2-alpha kinase 3 |
| Contents | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human PERK(163-176aa QWDQDRESMETVPF). |
| Purification | Immunogen affinity purified. |
| Cross Reactivity | No cross reactivity with other proteins |
| Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
| Sequence Similarities | Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily. |
| Name | EIF2AK3 |
|---|---|
| Synonyms | PEK, PERK |
| Function | Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) in response to various stress conditions. Key activator of the integrated stress response (ISR) required for adaptation to various stress, such as unfolded protein response (UPR) and low amino acid availability (By similarity). EIF2S1/eIF-2-alpha phosphorylation in response to stress converts EIF2S1/eIF-2-alpha in a global protein synthesis inhibitor, leading to a global attenuation of cap-dependent translation, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activators ATF4 and QRICH1, and hence allowing ATF4- and QRICH1-mediated reprogramming (PubMed:33384352). Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1). Involved in control of mitochondrial morphology and function (By similarity). |
| Cellular Location | Endoplasmic reticulum membrane; Single-pass type I membrane protein |
| Tissue Location | Ubiquitous. A high level expression is seen in secretory tissues |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
EIF2AK3(Eukaryotic Translation Initiation Factor 2-Alpha Kinase 3), also called PEK, is an enzyme that in humans is encoded by the EIF2AK3 gene. By fluorescence in situ hybridization and radiation hybrid analysis, Hayes et al.(1999) mapped the EIF2AK3 gene to chromosome 2p12. Harding et al.(2000) reported that a targeted mutation of the mouse Eif2ak3 gene, which they called Perk, abolished the phosphorylation of eIF2-alpha in response to accumulation of malfolded proteins in the ER, resulting in abnormally elevated protein synthesis and higher levels of ER stress. Using a library of endoribonuclease-prepared short interfering RNAs(esiRNAs), Kittler et al.(2004) identified 37 genes required for cell division, one of which was EIF2AK3.
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