Anti-ABCG4 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB |
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Primary Accession | Q9H172 |
Host | Rabbit |
Reactivity | Human, Mouse, Rat |
Clonality | Polyclonal |
Format | Lyophilized |
Description | Rabbit IgG polyclonal antibody for ATP-binding cassette sub-family G member 4(ABCG4) detection. Tested with WB in Human;Mouse;Rat. |
Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Gene ID | 64137 |
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Other Names | ATP-binding cassette sub-family G member 4, ABCG4, WHITE2 |
Calculated MW | 71896 MW KDa |
Application Details | Western blot, 0.1-0.5 µg/ml, Mouse, Rat, Human |
Subcellular Localization | Membrane ; Multi-pass membrane protein . |
Tissue Specificity | Highly expressed in brain tissues with the exception of the spinal cord. . |
Protein Name | ATP-binding cassette sub-family G member 4 |
Contents | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human ABCG4(327-341aa AVQNGLCAMAEKKSS), different from the related mouse and rat sequences by one amino acid. |
Purification | Immunogen affinity purified. |
Cross Reactivity | No cross reactivity with other proteins |
Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
Sequence Similarities | Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily. |
Name | ABCG4 (HGNC:13884) |
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Synonyms | WHITE2 |
Function | ATP-dependent transporter of the ATP-binding cassette (ABC) family that may be involved in the cellular efflux of sterols, in particular cholesterol and desmosterol (a cholesterol precursor), to high-density lipoprotein (HDL) (PubMed:15240127, PubMed:33141061). May play an important role in the removal of amyloid-beta peptides from brain, in a process that can be antagonized by desmosterol. However it is unclear whether ABCG4 can directly transport amyloid-beta peptides or whether peptide export may be facilitated due to changes in the membrane lipid environment (By similarity). Induces apoptosis in various cells (PubMed:27228027). |
Cellular Location | Cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane {ECO:0000250|UniProtKB:Q91WA9}; Multi-pass membrane protein. Endosome membrane {ECO:0000250|UniProtKB:Q91WA9}; Multi- pass membrane protein |
Tissue Location | Expressed specifically in the brain and the eye. |
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Provided below are standard protocols that you may find useful for product applications.
Background
ABCG4(ATP-Binding Cassette, Subfamily G, Member 4), is a protein that in humans is encoded by the ABCG4 gene. The protein encoded by this gene is included in the superfamily of ATP-binding cassette(ABC) transporters. This protein is a member of the White subfamily and is expressed predominantly in liver tissue. By genomic sequence analysis, Engel et al.(2001) mapped the ABCG4 gene to chromosome 11q23.3. Engel et al.(2001) demonstrated 5-fold induction of ABCG4 following treatment of normal monocyte-derived macrophages with the LXR and RXR agonists 9-cis retinoic acid and 22R hydroxycholesterol. Removal of cholesterol from macrophages by cyclodextrin decreased ABCG4 message levels.
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