Anti-CACNA1A Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB |
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Primary Accession | O00555 |
Host | Rabbit |
Reactivity | Human, Mouse, Rat |
Clonality | Polyclonal |
Format | Lyophilized |
Description | Rabbit IgG polyclonal antibody for Voltage-dependent P/Q-type calcium channel subunit alpha-1A(CACNA1A) detection. Tested with WB in Human;Mouse;Rat. |
Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Gene ID | 773 |
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Other Names | Voltage-dependent P/Q-type calcium channel subunit alpha-1A, Brain calcium channel I, BI, Calcium channel, L type, alpha-1 polypeptide isoform 4, Voltage-gated calcium channel subunit alpha Cav2.1, CACNA1A, CACH4, CACN3, CACNL1A4 |
Calculated MW | 282365 MW KDa |
Application Details | Western blot, 0.1-0.5 µg/ml, Rat, Human, Mouse |
Subcellular Localization | Membrane; Multi-pass membrane protein. |
Tissue Specificity | Brain specific; mainly found in cerebellum, cerebral cortex, thalamus and hypothalamus. Expressed in the small cell lung carcinoma cell line SCC-9. No expression in heart, kidney, liver or muscle. Purkinje cells contain predominantly P- type VSCC, the Q-type being a prominent calcium current in cerebellar granule cells. . |
Protein Name | Voltage-dependent P/Q-type calcium channel subunit alpha-1A |
Contents | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human CACNA1A(430-447aa KTDLLNPEEAEDQLADIA), identical to the related mouse and rat sequences. |
Purification | Immunogen affinity purified. |
Cross Reactivity | No cross reactivity with other proteins |
Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
Sequence Similarities | Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. CACNA1A subfamily. |
Name | CACNA1A (HGNC:1388) |
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Synonyms | CACH4, CACN3, CACNL1A4 |
Function | Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1A gives rise to P and/or Q- type calcium currents. P/Q-type calcium channels belong to the 'high- voltage activated' (HVA) group and are specifically blocked by the spider omega-agatoxin-IVA (AC P54282) (By similarity). They are however insensitive to dihydropyridines (DHP). |
Cellular Location | Cell membrane; Multi-pass membrane protein |
Tissue Location | Brain specific; mainly found in cerebellum, cerebral cortex, thalamus and hypothalamus. Expressed in the small cell lung carcinoma cell line SCC-9. No expression in heart, kidney, liver or muscle. Purkinje cells contain predominantly P-type VSCC, the Q-type being a prominent calcium current in cerebellar granule cells |
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Provided below are standard protocols that you may find useful for product applications.
Background
The CACNA1A gene, also known as CACNL1A4 or CaV2.1, encodes the transmembrane pore-forming subunit of the P/Q-type or CaV2.1 voltage-gated calcium channel(VGCC). Those channels not only mediate the entry of Ca(2+) ions into excitable cells but are also involved in a variety of Ca(2+)-dependent processes, including muscle contraction, hormone or neurotransmitter release, and gene expression. The channel activity is directed by the pore-forming alpha-1 subunit. CACNA1A is mapped to 19p13.2. This gene encodes the alpha-1A subunit, which is predominantly expressed in neuronal tissue. Mutations in this gene are associated with 2 neurologic disorders, familial hemiplegic migraine and episodic ataxia 2. This gene also exhibits polymorphic variation due to(CAG)n-repeats. CACNA1A has been shown to interact with CACNB4.
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