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Anti-CD40/TNFRSF5 Picoband Antibody

     
  • WB - Anti-CD40/TNFRSF5 Picoband Antibody ABO11670
    Western blot analysis of CD40/TNFRSF5 expression in mouse spleen extract (lane 1). CD40/TNFRSF5 at 40KD was detected using rabbit anti- CD40/TNFRSF5 Antigen Affinity purified polyclonal antibody (Catalog # ABO11670) at 0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method .
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  • IHC - Anti-CD40/TNFRSF5 Picoband Antibody ABO11670
    CD40/TNFRSF5 was detected in paraffin-embedded sections of mouse lymphaden tissues using rabbit anti- CD40/TNFRSF5 Antigen Affinity purified polyclonal antibody (Catalog # ABO11670) at 1 ??g/mL. The immunohistochemical section was developed using SABC method .
    detail
  • FC - Anti-CD40/TNFRSF5 Picoband Antibody ABO11670
    Figure 3. Flow Cytometry analysis of U937 cells using anti-CD40/TNFRSF5 antibody (ABO11670).Overlay histogram showing U937 cells stained with ABO11670 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD40/TNFRSF5 Antibody (ABO11670,1μg/1x106 cells) for 30 min at 20°C. DyLight?488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, FC, ICC, E
Primary Accession P27512
Host Rabbit
Reactivity Human, Mouse
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Tumor necrosis factor receptor superfamily member 5(CD40) detection. Tested with WB, IHC-P, IHC-F, ICC, ELISA, FCM in Human;Mouse.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 21939
Other Names Tumor necrosis factor receptor superfamily member 5, B-cell surface antigen CD40, Bp50, CD40L receptor, CD40, Cd40, Tnfrsf5
Calculated MW 32093 MW KDa
Application Details Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, By Heat
Immunohistochemistry(Frozen Section), 0.5-1 µg/ml

Immunocytochemistry, 0.5-1 µg/ml

ELISA , 0.1-0.5 µg/ml
Western blot, 0.1-0.5 µg/ml
Flow Cytometry, 1-3μg/1x106 cells
Subcellular Localization Isoform I: Cell membrane; Single-pass type I membrane protein.
Protein Name Tumor necrosis factor receptor superfamily member 5
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen E. coli-derived mouse CD40/TNFRSF5 recombinant protein (Position: L20-R193). Mouse CD40/TNFRSF5 shares 60.7% amino acid (aa) sequence identity with human CD40/TNFRSF5.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Protein Information
Name Cd40
Synonyms Tnfrsf5
Function Receptor for TNFSF5/CD40LG (By similarity). Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion (PubMed:12881420).
Cellular Location [Isoform I]: Cell membrane; Single-pass type I membrane protein [Isoform IV]: Cell membrane; Single-pass type I membrane protein [Isoform II]: Secreted.
Research Areas
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Background

Carbonic anhydrase III (CA3) is an enzyme that in humans is encoded by the CA3 gene. CA3 is a member of a multigene family (at least six separate genes are known) that encode carbonic anhydrase isozymes. The gene spans 10.3 kb and contains seven exons and six introns. Using a cDNA clone of the CA3 gene in the study of human-rodent hybrids, the gene was mapped to chromosome 8 which carries a cluster of CA genes. The expression of the CA3 gene is strictly tissue specific and present at high levels in skeletal muscle and much lower levels in cardiac and smooth muscle. A proportion of carriers of Duchenne muscle dystrophy have a higher CA3 level than normal.

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$ 280.00
Cat# ABO11670
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