Anti-CARD12 Picoband Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application ![]()
| WB |
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Primary Accession | Q9NPP4 |
Host | Rabbit |
Reactivity | Human, Mouse, Rat |
Clonality | Polyclonal |
Format | Lyophilized |
Description | Rabbit IgG polyclonal antibody for NLR family CARD domain-containing protein 4(NLRC4) detection. Tested with WB in Human;Mouse;Rat. |
Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Gene ID | 58484 |
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Other Names | NLR family CARD domain-containing protein 4, CARD, LRR, and NACHT-containing protein, Clan protein, Caspase recruitment domain-containing protein 12, Ice protease-activating factor, Ipaf, NLRC4, CARD12, CLAN, CLAN1, IPAF |
Calculated MW | 116159 MW KDa |
Application Details | Western blot, 0.1-0.5 µg/ml, Human, Mouse, Rat |
Subcellular Localization | Cytoplasm . Cytoplasm, cytosol . Cytoplasmic filaments. |
Tissue Specificity | Isoform 2 is expressed ubiquitously, although highly expressed in lung and spleen. Isoform 1 is highly expressed in lung, followed by leukocytes especially monocytes, lymph node, colon, brain, prostate, placenta, spleen, bone marrow and fetal liver. Isoform 4 is only detected in brain. |
Protein Name | NLR family CARD domain-containing protein 4 |
Contents | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human CARD12 (838-874aa KILAQNLHNLVKLSILDLSENYLEKDGNEALHELIDR), different from the related mouse sequence by five amino acids, and from the related rat sequence by seven amino acids. |
Purification | Immunogen affinity purified. |
Cross Reactivity | No cross reactivity with other proteins |
Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
Name | NLRC4 |
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Function | Key component of inflammasomes that indirectly senses specific proteins from pathogenic bacteria and fungi and responds by assembling an inflammasome complex that promotes caspase-1 activation, cytokine production and macrophage pyroptosis (PubMed:15107016). The NLRC4 inflammasome is activated as part of the innate immune response to a range of intracellular bacteria (By similarity). |
Cellular Location | Cytoplasm. Cytoplasm, cytosol {ECO:0000250|UniProtKB:Q3UP24}. Inflammasome |
Tissue Location | Isoform 2 is expressed ubiquitously, although highly expressed in lung and spleen. Isoform 1 is highly expressed in lung, followed by leukocytes especially monocytes, lymph node, colon, brain, prostate, placenta, spleen, bone marrow and fetal liver. Isoform 4 is only detected in brain |

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Provided below are standard protocols that you may find useful for product applications.
Background
NLR family CARD domain-containing protein 4, also known as CARD12, is a protein that in humans is encoded by the NLRC4 gene. It is mapped to 2p22.3. This gene encodes a member of the caspase recruitment domain-containing NLR family. Family members play essential roles in innate immune response to a wide range of pathogenic organisms, tissue damage and other cellular stresses. Mutations in this gene result in autoinflammation with infantile enterocolitis. Alternative splicing results in multiple transcript variants.

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