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Anti-human DDT Antibody

     
  • WB - Anti-human DDT Antibody ABO12696
    Figure. Western blot analysis of DDT using anti- DDT antibody (ABO12696). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane: Recombinant Human DDT Protein 0.5ngAfter Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- DDT antigen affinity purified polyclonal antibody (Catalog # ABO12696) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for DDT at approximately 15KD. The expected band size for DDT is at 15KD.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, ICC, E
Primary Accession P30046
Host Rabbit
Reactivity Human
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for D-dopachrome decarboxylase(DDT) detection. Tested with WB, IHC-P, ICC, ELISA in Human.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 1652
Other Names D-dopachrome decarboxylase, 4.1.1.84, D-dopachrome tautomerase, Phenylpyruvate tautomerase II, DDT
Calculated MW 12712 MW KDa
Application Details ELISA , 0.1-0.5 µg/ml, Human, -
Immunocytochemistry , 0.5-1 µg/ml, Human, -
Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, By Heat
Western blot, 0.1-0.5 µg/ml, Human
Subcellular Localization Cytoplasm .
Protein Name D-dopachrome decarboxylase
Contents Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Carrier free (No BSA) form available in stock. If you want this antibody carrier free please specify Carrier Free" or "No BSA" in your order note. "
Immunogen E. coli-derived human DDT recombinant protein(Position: M1-L118).
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Protein Information
Name DDT
Function Tautomerization of D-dopachrome with decarboxylation to give 5,6-dihydroxyindole (DHI).
Cellular Location Cytoplasm.
Tissue Location Highly expressed in the liver and at lower levels in the heart, lung and pancreas.
Research Areas
Citations (0)
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Background

DDT, D-dopachrome tautomerization, converts D-dopachrome into 5,6-dihydroxyindole. Northern blot analysis revealed that DDT was expressed as a 0.6-kb mRNA in all tissues tested, with the strongest expression in liver. The DDT gene in human and mouse is identical in exon structure to the MIF gene. Both genes have 2 introns that are located at equivalent positions, relative to a 2-fold repeat in protein structure.the genes for DDT and MIF are closely linked on human chromosome 22 and mouse chromosome 10.

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$ 370.00
Cat# ABO12696
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