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Anti-MBD2 Antibody
- SPECIFICATION
- CITATIONS
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- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | Q9UBB5 |
| Host | Rabbit |
| Reactivity | Human |
| Clonality | Polyclonal |
| Format | Lyophilized |
| Description | Rabbit IgG polyclonal antibody for Methyl-CpG-binding domain protein 2(MBD2) detection. Tested with WB in Human. |
| Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
| Gene ID | 8932 |
|---|---|
| Other Names | Methyl-CpG-binding domain protein 2, Demethylase, DMTase, Methyl-CpG-binding protein MBD2, MBD2 |
| Calculated MW | 43255 MW KDa |
| Application Details | Western blot, 0.1-0.5 µg/ml, Human |
| Subcellular Localization | Nucleus . Nuclear, in discrete foci. Detected at replication foci in late S phase. |
| Tissue Specificity | Highly expressed in brain, heart, kidney, stomach, testis and placenta. . |
| Protein Name | Methyl-CpG-binding domain protein 2 |
| Contents | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
| Immunogen | E.coli-derived human MBD2 recombinant protein (Position: W159-A411). Human MBD2 shares 98% amino acid (aa) sequence identity with mouse MBD2. |
| Purification | Immunogen affinity purified. |
| Cross Reactivity | No cross reactivity with other proteins |
| Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
| Sequence Similarities | Contains 1 MBD (methyl-CpG-binding) domain. |
| Name | MBD2 (HGNC:6917) |
|---|---|
| Function | Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides (PubMed:9774669). Binds hemimethylated DNA as well (PubMed:10947852, PubMed:24307175). Recruits histone deacetylases and DNA methyltransferases to chromatin (PubMed:10471499, PubMed:10947852). Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin (PubMed:16428440, PubMed:28977666). Acts as a transcriptional repressor and plays a role in gene silencing (PubMed:10471499, PubMed:10947852, PubMed:16415179). Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression (PubMed:16415179). May enhance the activation of some unmethylated cAMP-responsive promoters (PubMed:12665568). |
| Cellular Location | Nucleus. Chromosome Note=Nuclear, in discrete foci (PubMed:12183469). Detected at replication foci in late S phase. Localizes to methylated chromatin (PubMed:16428440). Localizes to sites of DNA damage in a manner partially dependent on ZMYND8 (PubMed:27732854) |
| Tissue Location | Highly expressed in brain, heart, kidney, stomach, testis and placenta. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Methyl-CpG-binding domain protein 2 is a protein that in humans is encoded by the MBD2 gene. It is mapped to 18q21.2. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG-binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing.
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