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Anti-ATP5A1/Atp5A Rabbit Monoclonal Antibody

     
  •  - Anti-ATP5A1/Atp5A Rabbit Monoclonal Antibody ABO13871
    All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
    detail
  •  - Anti-ATP5A1/Atp5A Rabbit Monoclonal Antibody ABO13871
    Immunofluorescent analysis using the Antibody at 1:50 dilution.
    detail
  •  - Anti-ATP5A1/Atp5A Rabbit Monoclonal Antibody ABO13871
    Immunofluorescent analysis using the Antibody at 1:50 dilution.
    detail
  •  - Anti-ATP5A1/Atp5A Rabbit Monoclonal Antibody ABO13871
    Western blot analysis of ATP5A1 expression in HepG2 cell lysate;.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF, ICC, FC
Primary Accession P25705
Host Rabbit
Isotype Rabbit IgG
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Format Liquid
Description Anti-ATP5A1/Atp5A Rabbit Monoclonal Antibody . Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Additional Information
Gene ID 498
Other Names ATP synthase subunit alpha, mitochondrial, ATP synthase F1 subunit alpha {ECO:0000312|HGNC:HGNC:823}, ATP5F1A (HGNC:823)
Calculated MW 59751 MW KDa
Application Details WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Subcellular Localization Mitochondrion inner membrane. Cell membrane; Peripheral membrane protein; Extracellular side. Colocalizes with HRG on the cell surface of T-cells (PubMed:19285951).
Tissue Specificity Fetal lung, heart, liver, gut and kidney. Expressed at higher levels in the fetal brain, retina and spinal cord..
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: AAFC-1
Immunogen A synthesized peptide derived from human ATP5A1
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Protein Information
Name ATP5F1A (HGNC:823)
Function Subunit alpha, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain (Probable). ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel (PubMed:37244256). These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk (PubMed:37244256). During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation (Probable). In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro (By similarity). With the catalytic subunit beta (ATP5F1B), forms the catalytic core in the F(1) domain (PubMed:37244256). Subunit alpha does not bear the catalytic high- affinity ATP-binding sites (Probable). Binds the bacterial siderophore enterobactin and can promote mitochondrial accumulation of enterobactin-derived iron ions (PubMed:30146159).
Cellular Location Mitochondrion. Mitochondrion inner membrane {ECO:0000250|UniProtKB:P19483}; Peripheral membrane protein {ECO:0000250|UniProtKB:P19483}; Matrix side {ECO:0000250|UniProtKB:P19483}. Cell membrane; Peripheral membrane protein; Extracellular side. Note=Colocalizes with HRG on the cell surface of T-cells (PubMed:19285951).
Tissue Location Fetal lung, heart, liver, gut and kidney. Expressed at higher levels in the fetal brain, retina and spinal cord
Research Areas
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$ 370.00
Cat# ABO13871
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