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Anti-JNK1/2/3 MAPK8 Rabbit Monoclonal Antibody

     
  •  - Anti-JNK1/2/3 MAPK8 Rabbit Monoclonal Antibody ABO13920
    Immunofluorescent analysis using the Antibody at 1:50 dilution.
    detail
  •  - Anti-JNK1/2/3 MAPK8 Rabbit Monoclonal Antibody ABO13920
    Immunofluorescent analysis of Hela cells, using JNK1/2/3 Antibody.
    detail
  •  - Anti-JNK1/2/3 MAPK8 Rabbit Monoclonal Antibody ABO13920
    Figure 1. Western blot analysis of JNK1/2/3 using anti-JNK1/2/3 antibody (M02608-1).
    Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human Hela whole cell lysates,
    Lane 2: human U87 whole cell lysates,
    Lane 3: human A549 whole cell lysates,
    Lane 4: rat PC-12 whole cell lysates,
    Lane 5: rat C6 whole cell lysates,
    Lane 6: mouse Neuro-2a whole cell lysates,
    Lane 7: mouse NIH/3T3 whole cell lysates.
    After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JNK1/2/3 antigen affinity purified monoclonal antibody (Catalog # M02608-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for JNK1/2/3 at approximately 40, 48 kDa. The expected band size for JNK1/2/3 is at 48 kDa.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IF, ICC, IP
Primary Accession P45983/P45984/P53779
Host Rabbit
Isotype Rabbit IgG
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Format Liquid
Description Anti-JNK1/2/3 MAPK8 Rabbit Monoclonal Antibody . Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Additional Information
Calculated MW 48296 MW KDa
Application Details WB 1:1000-1:2000
ICC/IF 1:50-1:200
IP 1:50
Subcellular Localization Cytoplasm. Nucleus.
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/ml BSA.
Clone Names Clone: DFI-13
Immunogen A synthesized peptide derived from human JNK1+JNK2+JNK3
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Protein Information
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$ 370.00
Cat# ABO13920
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