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Anti-Cardiac FABP Fabp3 Antibody Picoband™ (monoclonal, 6C4)

     
  • WB - Anti-Cardiac FABP Fabp3 Antibody Picoband™ (monoclonal, 6C4) ABO14334
    Figure 1. Western blot analysis of Cardiac FABP using anti-Cardiac FABP antibody (M01734).
    Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
    Lane 1: mouse heart tissue lysates,
    Lane 2: mouse heart tissue lysates,
    Lane 3: rat heart tissue lysates,
    Lane 4: rat heart tissue lysates.
    After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Cardiac FABP antigen affinity purified monoclonal antibody (Catalog # M01734) at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.
    detail
  • IHC - Anti-Cardiac FABP Fabp3 Antibody Picoband™ (monoclonal, 6C4) ABO14334
    Figure 2. IHC analysis of Cardiac FABP using anti-Cardiac FABP antibody (M01734).
    Cardiac FABP was detected in paraffin-embedded section of rat cardiac muscle tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Cardiac FABP Antibody (M01734) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
    detail
  • IHC - Anti-Cardiac FABP Fabp3 Antibody Picoband™ (monoclonal, 6C4) ABO14334
    Figure 3. Sandwich ELISA - Recombinant rat Cardiac FABP protein standard curve.
    Use in combination with reagents from Rat Cardiac FABP ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ1623).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, E
Primary Accession P07483
Host Mouse
Isotype Mouse IgG1
Reactivity Rat, Mouse
Clonality Monoclonal
Format Lyophilized
Description Anti-Cardiac FABP Fabp3 Antibody Picoband™ (monoclonal, 6C4) . Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 79131
Other Names Fatty acid-binding protein, heart, Fatty acid-binding protein 3, Heart-type fatty acid-binding protein, H-FABP, Fabp3
Calculated MW 15 kDa
Application Details Western blot, 0.1-0.5 µg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 µg/ml
ELISA (Cap), 1-5 µg/ml
Subcellular Localization Cytoplasm.
Tissue Specificity Heart, but also skeletal muscle, kidney, brain and mammary gland.
Contents Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clone Names Clone: 6C4
Immunogen E. coli-derived rat Cardiac FABP recombinant protein (Position: A2-A133).
Cross Reactivity No cross-reactivity with other proteins.
Storage Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Protein Information
Name Fabp3
Function FABPs are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters.
Cellular Location Cytoplasm.
Tissue Location Heart, but also skeletal muscle, kidney, brain and mammary gland
Research Areas
Citations (0)
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Background

Heart-type fatty acid binding protein (hFABP), also known as mammary-derived growth inhibitor, is a protein that in humans is encoded by the FABP3 gene. The intracellular fatty acid-binding proteins (FABPs) belong to a multigene family. Fatty acid-binding protein 3 gene contains four exons and its function is to arrest growth of mammary epithelial cells. This gene is also a candidate tumor suppressor gene for human breast cancer. Cardiac-type fatty acid-binding protein (cFABP) from human heart muscle of three individuals was isolated and characterized as pI 5.3-cFABP.

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$ 370.00
Cat# ABO14334
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