Anti-Nogo Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application ![]()
| WB, IHC, IF, ICC, IP, FC |
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Primary Accession | Q9NQC3 |
Host | Rabbit |
Isotype | Rabbit IgG |
Reactivity | Human |
Clonality | Monoclonal |
Format | Liquid |
Description | Anti-Nogo Monoclonal Antibody . Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human. |
Gene ID | 57142 |
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Other Names | Reticulon-4, Foocen, Neurite outgrowth inhibitor, Nogo protein, Neuroendocrine-specific protein, NSP, Neuroendocrine-specific protein C homolog, RTN-x, Reticulon-5, RTN4 (HGNC:14085) |
Calculated MW | 129931 Da |
Application Details | WB 1:500-1:2000 IHC 1:50-1:200 ICC/IF 1:50-1:200 IP 1:50 FC 1:50 |
Contents | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA. |
Clone Names | Clone: AEAH-18 |
Immunogen | A synthesized peptide derived from human Nogo Developmental neurite growth regulatory factor with a role as a negative regulator of axon-axon adhesion and growth, and as a facilitator of neurite branching. Regulates neurite fasciculation, branching and extension in the developing nervous system. |
Purification | Affinity-chromatography |
Storage | Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. |
Name | RTN4 (HGNC:14085) |
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Function | Required to induce the formation and stabilization of endoplasmic reticulum (ER) tubules (PubMed:24262037, PubMed:25612671, PubMed:27619977). They regulate membrane morphogenesis in the ER by promoting tubular ER production (PubMed:24262037, PubMed:25612671, PubMed:27619977, PubMed:27786289). They influence nuclear envelope expansion, nuclear pore complex formation and proper localization of inner nuclear membrane proteins (PubMed:26906412). However each isoform have specific functions mainly depending on their tissue expression specificities (Probable). |
Cellular Location | [Isoform A]: Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein; Cytoplasmic side Synapse {ECO:0000250|UniProtKB:Q99P72}. Note=Anchored to the membrane of the endoplasmic reticulum (ER) through 2 putative transmembrane domains. Localizes throughout the ER tubular network (PubMed:27619977) Co-localizes with TMEM33 at the ER sheets [Isoform C]: Endoplasmic reticulum membrane; Multi-pass membrane protein |
Tissue Location | Isoform A: is specifically expressed in brain and testis and weakly in heart and skeletal muscle. Isoform B: widely expressed except for the liver. Highly expressed in endothelial cells and vascular smooth muscle cells, including blood vessels and mesenteric arteries (PubMed:15034570, PubMed:21183689). Isoform C: is expressed in brain, skeletal muscle and adipocytes. Isoform D is testis-specific. |

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