Anti-PPT1 Antibody Picoband™ (monoclonal, 10F3)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application ![]()
| WB, IHC, FC |
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Primary Accession | P50897 |
Host | Mouse |
Isotype | Mouse IgG2b |
Reactivity | Human |
Clonality | Monoclonal |
Format | Lyophilized |
Description | Anti-PPT1 Antibody Picoband™ (monoclonal, 10F3) . Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human. |
Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500 µg/ml. |
Gene ID | 5538 |
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Other Names | Palmitoyl-protein thioesterase 1, PPT-1, 3.1.2.22, Palmitoyl-protein hydrolase 1, PPT1, CLN1 {ECO:0000303|PubMed:19941651}, PPT |
Calculated MW | 34 kDa |
Application Details | Western blot, 0.1-0.5 µg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1 µg/ml Flow Cytometry, 1-3 µg/1x10^6 cells |
Subcellular Localization | Lysosome. Secreted. |
Protein Name | Palmitoyl-protein thioesterase 1 |
Contents | Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
Clone Names | Clone: 10F3 |
Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human PPT1, different from the related mouse and rat sequences by four amino acids. |
Cross Reactivity | No cross-reactivity with other proteins. |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Name | PPT1 |
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Synonyms | CLN1 {ECO:0000303|PubMed:19941651}, PPT |
Function | Has thioesterase activity against fatty acid thioesters with 14 -18 carbons, including palmitoyl-CoA, S-palmitoyl-N- acetylcysteamine, and palmitoylated proteins (PubMed:12855696, PubMed:26731412, PubMed:8816748). In contrast to PPT2, PPT1 can hydrolyze palmitoylated proteins and palmitoylcysteine (PubMed:12855696). |
Cellular Location | Lysosome. Secreted Golgi apparatus. Endoplasmic reticulum |

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Background
Palmitoyl-protein thioesterase 1 (PPT-1), also known as palmitoyl-protein hydrolase 1, is an enzyme that in humans is encoded by the PPT1 gene. PPT-1 is a member of the palmitoyl protein thioesterase family. The protein encoded by this gene is a small glycoprotein involved in the catabolism of lipid-modified proteins during lysosomal degradation. The encoded enzyme removes thioester-linked fatty acyl groups such as palmitate from cysteine residues. Defects in this gene are a cause of infantile neuronal ceroid lipofuscinosis 1 (CLN1, or INCL) and neuronal ceroid lipofuscinosis 4 (CLN4). Two transcript variants encoding different isoforms have been found for this gene.

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