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Anti-HSPB8/Hsp22 Antibody Picoband™ (monoclonal, 7D8)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IF, ICC, FC |
|---|---|
| Primary Accession | Q9UJY1 |
| Host | Mouse |
| Isotype | Mouse IgG2b |
| Reactivity | Rat, Human |
| Clonality | Monoclonal |
| Format | Lyophilized |
| Description | Anti-HSPB8/Hsp22 Antibody Picoband™ (monoclonal, 7D8) . Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Rat. |
| Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
| Gene ID | 26353 |
|---|---|
| Other Names | Heat shock protein beta-8, HspB8, Alpha-crystallin C chain, E2-induced gene 1 protein, Heat shock protein family B member 8, Protein kinase H11, Small stress protein-like protein HSP22, HSPB8, CRYAC, E2IG1, HSP22 |
| Calculated MW | 22 kDa |
| Application Details | Western blot, 0.1-0.5 µg/ml, Human, Rat Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human Flow Cytometry, 1-3 µg/1x10^6 cells, Human |
| Subcellular Localization | Nucleus. Cytoplasm. |
| Tissue Specificity | Predominantly expressed in skeletal muscle and heart. |
| Contents | Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
| Clone Names | Clone: 7D8 |
| Immunogen | E.coli-derived human HSPB8/Hsp22 recombinant protein (Position: M1-T196). Human HSPB8/Hsp22 shares 94.4% and 95.4% amino acid (aa) sequence identity with mouse and rat HSPB8/Hsp22, respectively. |
| Purification | Immunogen affinity purified. |
| Cross Reactivity | No cross-reactivity with other proteins. |
| Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
| Name | HSPB8 |
|---|---|
| Synonyms | CRYAC, E2IG1, HSP22 |
| Function | Displays temperature-dependent chaperone activity. |
| Cellular Location | Cytoplasm. Nucleus Note=Translocates to nuclear foci during heat shock |
| Tissue Location | Predominantly expressed in skeletal muscle and heart. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Heat shock protein beta-8 is a protein that in humans is encoded by the HSPB8 gene. The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The expression of this gene in induced by estrogen in estrogen receptor-positive breast cancer cells, and this protein also functions as a chaperone in association with Bag3, a stimulator of macroautophagy. Thus, this gene appears to be involved in regulation of cell proliferation, apoptosis, and carcinogenesis, and mutations in this gene have been associated with different neuromuscular diseases, including Charcot-Marie-Tooth disease.
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