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Anti-Cytochrome p450 2C19/CYP2C19 Antibody Picoband™ (monoclonal, 10G5)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC, FC |
|---|---|
| Primary Accession | P33261 |
| Host | Mouse |
| Isotype | Mouse IgG1 |
| Reactivity | Rat, Human, Mouse, Monkey |
| Clonality | Monoclonal |
| Format | Lyophilized |
| Description | Anti-Cytochrome p450 2C19/CYP2C19 Antibody Picoband™ (monoclonal, 10G5) . Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. |
| Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
| Gene ID | 1557 |
|---|---|
| Other Names | Cytochrome P450 2C19, 1.14.14.1, (R)-limonene 6-monooxygenase, 1.14.14.53, (S)-limonene 6-monooxygenase, 1.14.14.51, (S)-limonene 7-monooxygenase, 1.14.14.52, CYPIIC17, CYPIIC19, Cytochrome P450-11A, Cytochrome P450-254C, Fenbendazole monooxygenase (4'-hydroxylating), 1.14.14.75, Mephenytoin 4-hydroxylase, CYP2C19 |
| Calculated MW | 55 kDa |
| Application Details | Western blot, 0.1-0.5 µg/ml, Mouse, Rat, Monkey Immunohistochemistry (Paraffin-embedded Section), 0.5-1 µg/ml, Human Flow Cytometry, 1-3 µg/1x10^6 cells, Human |
| Contents | Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3. |
| Clone Names | Clone: 10G5 |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human Cytochrome p450 2C19/CYP2C19. |
| Purification | Immunogen affinity purified. |
| Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
| Name | CYP2C19 |
|---|---|
| Function | A cytochrome P450 monooxygenase involved in the metabolism of polyunsaturated fatty acids (PUFA) (PubMed:18577768, PubMed:19965576, PubMed:20972997). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) (PubMed:18577768, PubMed:19965576, PubMed:20972997). Catalyzes the hydroxylation of carbon-hydrogen bonds. Hydroxylates PUFA specifically at the omega-1 position (PubMed:18577768). Catalyzes the epoxidation of double bonds of PUFA (PubMed:19965576, PubMed:20972997). Also metabolizes plant monoterpenes such as limonene. Oxygenates (R)- and (S)-limonene to produce carveol and perillyl alcohol (PubMed:11950794). Responsible for the metabolism of a number of therapeutic agents such as the anticonvulsant drug S-mephenytoin, omeprazole, proguanil, certain barbiturates, diazepam, propranolol, citalopram and imipramine. Hydroxylates fenbendazole at the 4' position (PubMed:23959307). |
| Cellular Location | Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein |
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Provided below are standard protocols that you may find useful for product applications.
Background
Cytochrome P450 2C19 (abbreviatedCYP2C19) encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is known to metabolize many xenobiotics, including the anticonvulsive drug mephenytoin, omeprazole, diazepam and some barbiturates. Polymorphism within this gene is associated with variable ability to metabolize mephenytoin, known as the poor metabolizer and extensive metabolizer phenotypes. The gene is located within a cluster of cytochrome P450 genes on chromosome 10q24.
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