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Anti-Gli3 Rabbit Monoclonal Antibody
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- BACKGROUND
Application 
| WB, IF, ICC |
|---|---|
| Primary Accession | P10071 |
| Host | Rabbit |
| Isotype | IgG |
| Reactivity | Human |
| Clonality | Monoclonal |
| Format | Liquid |
| Description | Anti-Gli3 Rabbit Monoclonal Antibody . Tested in WB, ICC/IF applications. This antibody reacts with Human. |
| Gene ID | 2737 |
|---|---|
| Other Names | Transcriptional activator GLI3, GLI3 form of 190 kDa, GLI3-190, GLI3 full-length protein, GLI3FL, Transcriptional repressor GLI3R, GLI3 C-terminally truncated form, GLI3 form of 83 kDa, GLI3-83, GLI3 |
| Calculated MW | 190 kDa |
| Application Details | WB 1:500-1:2000 ICC/IF 1:50-1:200 |
| Contents | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA. |
| Clone Names | Clone: 19G77 |
| Immunogen | A synthesized peptide derived from human Gli3 |
| Purification | Affinity-chromatography |
| Storage | Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. |
| Name | GLI3 |
|---|---|
| Function | Has a dual function as a transcriptional activator and a repressor of the sonic hedgehog (Shh) pathway, and plays a role in limb development. The full-length GLI3 form (GLI3FL) after phosphorylation and nuclear translocation, acts as an activator (GLI3A) while GLI3R, its C-terminally truncated form, acts as a repressor. A proper balance between the GLI3 activator and the repressor GLI3R, rather than the repressor gradient itself or the activator/repressor ratio gradient, specifies limb digit number and identity. In concert with TRPS1, plays a role in regulating the size of the zone of distal chondrocytes, in restricting the zone of PTHLH expression in distal cells and in activating chondrocyte proliferation. Binds to the minimal GLI- consensus sequence 5'-GGGTGGTC-3'. |
| Cellular Location | Nucleus. Cytoplasm {ECO:0000250|UniProtKB:Q61602}. Cell projection, cilium. Note=GLI3FL is localized predominantly in the cytoplasm while GLI3R resides mainly in the nucleus (By similarity). Ciliary accumulation requires the presence of KIF7 and SMO (PubMed:19592253). Translocation to the nucleus is promoted by interaction with ZIC1 (PubMed:11238441). TMEM216 reduces its nuclear localization (By similarity). {ECO:0000250|UniProtKB:Q61602, ECO:0000269|PubMed:11238441, ECO:0000269|PubMed:19592253} |
| Tissue Location | Is expressed in a wide variety of normal adult tissues, including lung, colon, spleen, placenta, testis, and myometrium. |
Research Areas
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Application Protocols
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$ 185.00
⚠️ Limit: 5 vials per order for discount.
Cat# ABO15401
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