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Anti-Bub3 Rabbit Monoclonal Antibody
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- BACKGROUND
Application 
| WB, IHC, IF, ICC, IP, FC |
|---|---|
| Primary Accession | O43684 |
| Host | Rabbit |
| Isotype | IgG |
| Reactivity | Rat, Human, Mouse |
| Clonality | Monoclonal |
| Format | Liquid |
| Description | Anti-Bub3 Rabbit Monoclonal Antibody . Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. |
| Gene ID | 9184 |
|---|---|
| Other Names | Mitotic checkpoint protein BUB3, BUB3 |
| Calculated MW | 40 kDa |
| Application Details | WB 1:1000-1:5000 IHC 1:50-1:200 ICC/IF 1:100-1:500 IP 1:50 FC 1:50 |
| Contents | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA. |
| Clone Names | Clone: 19B93 |
| Immunogen | A synthesized peptide derived from human Bub3 |
| Purification | Affinity-chromatography |
| Storage | Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. |
| Name | BUB3 |
|---|---|
| Function | Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. |
| Cellular Location | Nucleus. Chromosome, centromere, kinetochore. Note=Starts to localize at kinetochores in prometaphase I (Pro-MI) stage and maintains the localization until the metaphase I- anaphase I (MI-AI) transition. |
Research Areas
Citations (0)
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info@abcepta.com, and receive a free "I Love Antibodies" mug.
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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$ 370.00
Cat# ABO15417
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