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Anti-S1PR3 Rabbit Monoclonal Antibody

     
  •  - Anti-S1PR3 Rabbit Monoclonal Antibody ABO15826
    Figure 1. Western blot analysis of S1PR3 using anti-S1PR3 antibody (M03755).
    Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human HepG2 whole cell lysates,
    Lane 2: human Hela whole cell lysates,
    Lane 3: human A549 whole cell lysates,
    Lane 4: human SiHa whole cell lysates,
    Lane 5: rat C6 whole cell lysates,
    Lane 6: rat NRK whole cell lysates.
    After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-S1PR3 antigen affinity purified monoclonal antibody (Catalog # M03755) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for S1PR3 at approximately 42 kDa. The expected band size for S1PR3 is at 42 kDa.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q99500
Host Rabbit
Isotype IgG
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Format Liquid
Description Anti-S1PR3 Rabbit Monoclonal Antibody . Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Additional Information
Gene ID 1903
Other Names Sphingosine 1-phosphate receptor 3, S1P receptor 3, S1P3, Endothelial differentiation G-protein coupled receptor 3, Sphingosine 1-phosphate receptor Edg-3, S1P receptor Edg-3, S1PR3 (HGNC:3167)
Calculated MW 42 kDa
Application Details WB 1:500-1:2000
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: 24S03
Immunogen A synthesized peptide derived from human S1PR3
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Protein Information
Name S1PR3 (HGNC:3167)
Function Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. When expressed in rat HTC4 hepatoma cells, is capable of mediating S1P-induced cell proliferation and suppression of apoptosis.
Cellular Location Cell membrane; Multi-pass membrane protein.
Tissue Location Expressed in all tissues, but most abundantly in heart, placenta, kidney, and liver
Research Areas
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$ 370.00
Cat# ABO15826
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