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Anti-UFM1 Rabbit Monoclonal Antibody

     
  •  - Anti-UFM1 Rabbit Monoclonal Antibody ABO16306
    Western blot analysis of UFM1 expression in HepG2 cell lysate.
    detail
  •  - Anti-UFM1 Rabbit Monoclonal Antibody ABO16306
    Immunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:500 dilution)
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IP
Primary Accession P61960
Host Rabbit
Isotype IgG
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Format Liquid
Description Anti-UFM1 Rabbit Monoclonal Antibody . Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.
Additional Information
Gene ID 51569
Other Names Ubiquitin-fold modifier 1, UFM1 {ECO:0000303|PubMed:15071506, ECO:0000312|HGNC:HGNC:20597}
Calculated MW 10 kDa
Application Details WB 1:500-1:2000
IHC 1:50-1:200
IP 1:50
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: 28U42
Immunogen A synthesized peptide derived from human UFM1
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Protein Information
Name UFM1 {ECO:0000303|PubMed:15071506, ECO:0000312|HGNC:HGNC:20597}
Function Ubiquitin-like modifier which can be covalently attached via an isopeptide bond to lysine residues of substrate proteins as a monomer or a lysine-linked polymer (PubMed:15071506, PubMed:20018847, PubMed:27653677, PubMed:29868776, PubMed:30626644, PubMed:38377992, PubMed:38383785). The so-called ufmylation, requires the UFM1- activating E1 enzyme UBA5, the UFM1-conjugating E2 enzyme UFC1, and the UFM1-ligase E3 enzyme UFL1 (PubMed:15071506, PubMed:20018847, PubMed:27653677, PubMed:29868776). Ufmylation is involved in various processes, such as ribosome recycling, response to DNA damage, transcription or reticulophagy (also called ER-phagy) induced in response to endoplasmic reticulum stress (PubMed:25219498, PubMed:32160526, PubMed:38383785).
Cellular Location Nucleus. Cytoplasm
Research Areas
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$ 370.00
Cat# ABO16306
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