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Caspase-5 Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | P51878 |
| Other Accession | NP_004338 |
| Reactivity | Human, Mouse, Rat, Hamster, Monkey, Bovine |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 49736 Da |
| Gene ID | 838 |
|---|---|
| Application & Usage | Western blotting (0.5-4ag/ml). However, the optimal conditions should be determined individually. The affinity purified antibody preferentially detects the intermediate (32 kDa) and the fully (20 kDa) cleaved caspase-5. |
| Other Names | CASP-5, ICH-3 protease, TY protease, ICH3 |
| Target/Specificity | Caspase-5 |
| Antibody Form | Liquid |
| Appearance | Colorless liquid |
| Formulation | 100 µg (0.2 mg/ml) affinity purified rabbit anti-caspase-5 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA and 0.01% thimerosal. |
| Handling | The antibody solution should be gently mixed before use. |
| Reconstitution & Storage | -20 °C |
| Background Descriptions | |
| Precautions | Caspase-5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CASP5 {ECO:0000303|PubMed:16893518, ECO:0000312|HGNC:HGNC:1506} |
|---|---|
| Function | Thiol protease that acts as a mediator of programmed cell death (PubMed:28314590, PubMed:29898893). Initiates pyroptosis, a programmed lytic cell death pathway through cleavage of Gasdermin-D (GSDMD): cleavage releases the N-terminal gasdermin moiety (Gasdermin- D, N-terminal) that binds to membranes and forms pores, triggering pyroptosis (PubMed:29898893). Also mediates cleavage and maturation of IL18 (PubMed:37993714). Cleavage of GSDMD and IL18 is not strictly dependent on the consensus cleavage site but depends on an exosite interface on CASP4 (PubMed:37993714). During non-canonical inflammasome activation, cuts CGAS and may play a role in the regulation of antiviral innate immune activation (PubMed:28314590). |
| Tissue Location | Expressed in barely detectable amounts in most tissues except brain, highest levels being found in lung, liver and skeletal muscle. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Caspase family of cysteine proteases has been shown to play a key role in apoptosis. Caspase-5 is also known as Ich-3 and ICErel-III and can proteolytically processed by granzyme B. Over expression of caspase-5 induces apoptosis in Rat-1 and HeLa cells, which can be inhibited by CrmA and Bcl-2.
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