HK2 (Hexokinase II) (NT) Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC, E |
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Primary Accession | P52789 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 102380 Da |
Gene ID | 3099 |
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Positive Control | Western Blot: A375 cell lysate Immunohistochemistry: Human skeletal muscle tissue |
Application & Usage | The antibody can be used for ELISA (0.25 µg/ml), Western blotting (0.5 – 2.5 µg/ml) and Immunohistochemistry (2.5 – 5.0 µg/ml). |
Other Names | Hexokinase type II, HK II, Muscle form hexokinase |
Target/Specificity | HK2 (Hexokinase II) |
Antibody Form | Liquid |
Appearance | Colorless liquid |
Formulation | 100 µg (0.25 mg/ml) purified rabbit Ig polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. |
Handling | The antibody solution should be gently mixed before use. |
Reconstitution & Storage | -20 °C |
Background Descriptions | |
Precautions | HK2 (Hexokinase II) (NT) Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | HK2 (HGNC:4923) |
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Function | Catalyzes the phosphorylation of hexose, such as D-glucose and D-fructose, to hexose 6-phosphate (D-glucose 6-phosphate and D- fructose 6-phosphate, respectively) (PubMed:23185017, PubMed:26985301, PubMed:29298880). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (PubMed:29298880). Plays a key role in maintaining the integrity of the outer mitochondrial membrane by preventing the release of apoptogenic molecules from the intermembrane space and subsequent apoptosis (PubMed:18350175). |
Cellular Location | Mitochondrion outer membrane; Peripheral membrane protein. Cytoplasm, cytosol Note=The mitochondrial-binding peptide (MBP) region promotes association with the mitochondrial outer membrane (PubMed:29298880) The interaction with the mitochondrial outer membrane via the mitochondrial-binding peptide (MBP) region promotes higher stability of the protein (PubMed:29298880). Release from the mitochondrial outer membrane into the cytosol induces permeability transition pore (PTP) opening and apoptosis (PubMed:18350175). |
Tissue Location | Predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle |
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Provided below are standard protocols that you may find useful for product applications.
Background
In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III, and IV. Hexokinase is an allosteric enzyme inhibited by its product GLC-6-P. Hexokinase activity is involved in the first step in several metabolic pathways. HK3 is bound to the outer mitochondrial membrane. Its hydrophobic N-terminal sequence may be involved in membrane bindng. It is the predominant hexokinase isozyme expressed in insuline-responsive tissues such as skeletal muscle. The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated wiht the N-terminus. Altho µgh found in NIDDM patients, genetic variations of HK2 do not contribute to the disease.
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