CD40L Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB |
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Primary Accession | P27548 |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 29370 Da |
Gene ID | 21947 |
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Application & Usage | Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Jurkat cell lysate can be used as a positive control. |
Other Names | CD40L, CD40 Ligand, CD40Ligand, CD40-L, CD40-Ligand |
Target/Specificity | CD40L |
Antibody Form | Liquid |
Appearance | Colorless liquid |
Formulation | 100 µg (0.5 mg/ml) Protein A affinity purified rabbit anti-mouse CD40L polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA and 0.01% thimerosal. |
Handling | The antibody solution should be gently mixed before use. |
Reconstitution & Storage | -20 °C |
Background Descriptions | |
Precautions | CD40L Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | Cd40lg |
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Synonyms | Cd40l, Tnfsf5 |
Function | Cytokine that acts as a ligand to CD40/TNFRSF5 (By similarity). Costimulates T-cell proliferation and cytokine production (By similarity). Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation (By similarity). Induces the activation of NF-kappa-B (By similarity). Induces the activation of kinases MAPK8 and PAK2 in T-cells (By similarity). Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4 (PubMed:1374165). Involved in immunoglobulin class switching (PubMed:1374165). |
Cellular Location | Cell membrane {ECO:0000250|UniProtKB:P29965}; Single-pass type II membrane protein {ECO:0000250|UniProtKB:P29965} Cell surface {ECO:0000250|UniProtKB:P29965} |
Tissue Location | Specifically expressed on activated CD4+ T- lymphocytes |
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Provided below are standard protocols that you may find useful for product applications.
Background
CD40, also known as bp50 or CDW40, is a type I membrane protein found on the surface of B cells and primary carcinomas. It belongs to the TNF-R family. CD40 serves as the receptor for CD40 ligand (CD40L), a cytokine expressed on the cell surface of T cells. CD40 plays a critical role in B cell proliferation, antibody class switching, modulation of apoptosis in the germinal center thro µgh interaction with T cells expressing CD40L, and activation of CD4+ T cells. Ligation of CD40 with its ligand preferentially induces stress-activated protein kinases in B lymphocytes.
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