Goat Anti-ORAI1 / CRACM1 Antibody
Peptide-affinity purified goat antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Primary Accession | Q96D31 |
Other Accession | NP_116179, 84876, 109305 (mouse) |
Reactivity | Human |
Predicted | Pig, Dog |
Host | Goat |
Clonality | Polyclonal |
Concentration | 100ug/200ul |
Isotype | IgG |
Calculated MW | 32668 Da |
Gene ID | 84876 |
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Other Names | Calcium release-activated calcium channel protein 1, Protein orai-1, Transmembrane protein 142A, ORAI1, CRACM1, TMEM142A |
Format | 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | Goat Anti-ORAI1 / CRACM1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | ORAI1 |
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Synonyms | CRACM1, TMEM142A |
Function | Ca(2+) release-activated Ca(2+) (CRAC) channel subunit which mediates Ca(2+) influx following depletion of intracellular Ca(2+) stores and channel activation by the Ca(2+) sensor, STIM1 (PubMed:16582901, PubMed:16645049, PubMed:16733527, PubMed:16766533, PubMed:16807233, PubMed:19249086, PubMed:23307288, PubMed:24351972, PubMed:24591628, PubMed:28219928, PubMed:20354224, PubMed:26956484). CRAC channels are the main pathway for Ca(2+) influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT (PubMed:16582901). Plays a prominent role in Ca(2+) influx at the basolateral membrane of mammary epithelial cells independently of the Ca(2+) content of endoplasmic reticulum or Golgi stores. May mediate transepithelial transport of large quantities of Ca(2+) for milk secretion. |
Cellular Location | Cell membrane; Multi-pass membrane protein. Basolateral cell membrane {ECO:0000250|UniProtKB:Q8BWG9}; Multi-pass membrane protein. Note=Isoform beta is more mobile in the plasma membrane (PubMed:23307288). Colocalizes with STIM1 at the cell membrane (PubMed:27185316). |
Tissue Location | Expressed in naive CD4 and CD8 T cells (at protein level) (PubMed:26956484). Expressed at similar levels in naive and effector T helper cells (PubMed:20354224) |
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Provided below are standard protocols that you may find useful for product applications.
Background
CRACM1 is a plasma membrane protein essential for store-operated calcium entry (Vig et al., 2006 [PubMed 16645049]).
References
Protein kinase C-induced phosphorylation of Orai1 regulates the intracellular Ca2+ level via the store-operated Ca2+ channel. Kawasaki T, et al. J Biol Chem, 2010 Aug 13. PMID 20534587.
A novel native store-operated calcium channel encoded by Orai3: selective requirement of Orai3 versus Orai1 in estrogen receptor-positive versus estrogen receptor-negative breast cancer cells. Motiani RK, et al. J Biol Chem, 2010 Jun 18. PMID 20395295.
Differential redox regulation of ORAI ion channels: a mechanism to tune cellular calcium signaling. Bogeski I, et al. Sci Signal, 2010 Mar 30. PMID 20354224.
Pore architecture of the ORAI1 store-operated calcium channel. Zhou Y, et al. Proc Natl Acad Sci U S A, 2010 Mar 16. PMID 20194792.
Expression and association of TRPC subtypes with Orai1 and STIM1 in human parathyroid. Lu M, et al. J Mol Endocrinol, 2010 May. PMID 20194530.
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