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RAD51D / RAD51L3 Antibody (N-Term)

Peptide-affinity purified goat antibody

     
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
E
Primary Accession O75771
Other Accession NP_002869.3, NP_598332.1, NP_001136043.1, 5892
Predicted Human
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 35049 Da
Additional Information
Gene ID 5892
Other Names DNA repair protein RAD51 homolog 4, R51H3, RAD51 homolog D, RAD51-like protein 3, TRAD, RAD51D, RAD51L3
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRAD51D / RAD51L3 Antibody (N-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RAD51D
Synonyms RAD51L3
Function Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. Bind to single-stranded DNA (ssDNA) and has DNA-dependent ATPase activity. Part of the RAD51 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. Involved in telomere maintenance. The BCDX2 subcomplex XRCC2:RAD51D can stimulate Holliday junction resolution by BLM.
Cellular Location Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Chromosome, telomere
Tissue Location Expressed in colon, prostate, spleen, testis, ovary, thymus and small intestine. Weakly expressed in leukocytes
Research Areas
Citations (0)
citation

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Background

This antibody is expected to recognise all three human isoforms of this protein.

References

Identification, characterization, and genetic mapping of Rad51d, a new mouse and human RAD51/RecA-related gene. Pittman DL, Weinberg LR, Schimenti JC. Genomics. 1998 Apr 1;49(1):103-11. PMID: 9570954

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$ 216.00
Cat# AF2289a
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