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RUFY1 Antibody (internal region)
Peptide-affinity purified goat antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q96T51 |
| Other Accession | NP_079434.3, NP_001035541.1, 80230, 216724 (mouse), 360521 (rat) |
| Reactivity | Human |
| Predicted | Mouse, Rat, Dog |
| Host | Goat |
| Clonality | Polyclonal |
| Concentration | 0.5 mg/ml |
| Isotype | IgG |
| Calculated MW | 79818 Da |
| Gene ID | 80230 |
|---|---|
| Other Names | RUN and FYVE domain-containing protein 1, FYVE-finger protein EIP1, La-binding protein 1, Rab4-interacting protein, Zinc finger FYVE domain-containing protein 12, RUFY1, RABIP4, ZFYVE12 |
| Dilution | WB~~1:1000 E~~N/A |
| Format | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | RUFY1 Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | RUFY1 (HGNC:19760) |
|---|---|
| Synonyms | RABIP4, ZFYVE12 |
| Function | Activating adapter involved in cargo sorting from early/recycling endosomes. Regulates retrieval of proteins from endosomes to the trans-Golgi network through interaction with the dynein-dynactin complex (PubMed:36282215). Dual effector of RAB4B and RAB14, mediates a cooperative interaction allowing endosomal tethering and fusion (PubMed:20534812). Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in early endosomal trafficking (PubMed:14617813). In oocytes, self-assembles to form a protein matrix which hold together endolysosomes, autophagosomes and proteasomes and generate non-membrane-bound compartments called endo- lysosomal vesicular assemblies (ELVAs). In immature oocytes, ELVAs sequester ubiquitinated protein aggregates and degrade them upon oocyte maturation (By similarity). |
| Cellular Location | Early endosome membrane; Peripheral membrane protein. Note=In oocytes, localizes in non-membrane-bound compartments called endo-lysosomal vesicular assemblies (ELVAs). {ECO:0000250|UniProtKB:Q8BIJ7} |
| Tissue Location | Broadly expressed, with highest levels in lung, testis, kidney and brain. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
This antibody is expected to recognize both reported isoforms (NP_079434.3; NP_001035541.1). Reported variants represent identical protein: NP_001035542.1, NP_001035541.1.
References
ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage. Matsuoka S, Ballif BA, Smogorzewska A, McDonald ER, Hurov KE, Luo J, Bakalarski CE, Zhao Z, Solimini N, Lerenthal Y, Shiloh Y, Gygi SP, Elledge SJ, Science (New York, N.Y.) 2007 May 316 (5828): 1160-6. PMID: 17525332
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