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CYB5R3 / Dia 1 Antibody (C-Term)
Peptide-affinity purified goat antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC, E |
|---|---|
| Primary Accession | P00387 |
| Other Accession | NP_000389.1, NP_001123291.1, NP_001165131.1, 1727 |
| Reactivity | Human |
| Host | Goat |
| Clonality | Polyclonal |
| Concentration | 0.5 mg/ml |
| Isotype | IgG |
| Calculated MW | 34235 Da |
| Gene ID | 1727 |
|---|---|
| Other Names | NADH-cytochrome b5 reductase 3, B5R, Cytochrome b5 reductase, 1.6.2.2, Diaphorase-1, NADH-cytochrome b5 reductase 3 membrane-bound form, NADH-cytochrome b5 reductase 3 soluble form, CYB5R3, DIA1 |
| Dilution | WB~~1:1000 IHC~~1:100~500 E~~N/A |
| Format | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | CYB5R3 / Dia 1 Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CYB5R3 (HGNC:2873) |
|---|---|
| Synonyms | DIA1 |
| Function | Catalyzes the reduction of two molecules of cytochrome b5 using NADH as the electron donor. |
| Cellular Location | [Isoform 1]: Endoplasmic reticulum membrane; Lipid-anchor {ECO:0000250|UniProtKB:P20070}; Cytoplasmic side {ECO:0000250|UniProtKB:P20070}. Mitochondrion outer membrane; Lipid-anchor {ECO:0000250|UniProtKB:P20070}; Cytoplasmic side {ECO:0000250|UniProtKB:P20070} |
| Tissue Location | [Isoform 2]: Expressed at late stages of erythroid maturation. |
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Provided below are standard protocols that you may find useful for product applications.
Background
This antibody is expected to recognize all three reported isoforms (NP_000389.1; NP_001123291.1; NP_001165131.1).
References
RhoB regulates endosome transport by promoting actin assembly on endosomal membranes through Dia1. Fernandez-Borja M, Janssen L, Verwoerd D, Hordijk P, Neefjes J, J. Cell. Sci. 2005 Jun 118 (Pt 12): 2661-70. PMID: 15944396
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