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SDF4 (aa161-175) Antibody (internal region)

Peptide-affinity purified goat antibody

     
  • WB - SDF4 (aa161-175) Antibody (internal region) AF3472a
    AF3472a (0.1 µg/ml) staining of Jurkat lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
    detail
  • IHC - SDF4 (aa161-175) Antibody (internal region) AF3472a
    AF3472a (5 µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
    detail
  • IHC - SDF4 (aa161-175) Antibody (internal region) AF3472a
    EB10744 (5µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
    detail
  • WB - SDF4 (aa161-175) Antibody (internal region) AF3472a
    EB10744 (0.1µg/ml) staining of Jurkat (A), HeLa (B) and U251 (C) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
    detail
  • IF - SDF4 (aa161-175) Antibody (internal region) AF3472a
    EB10744 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing Golgi and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
    detail
  • FC - SDF4 (aa161-175) Antibody (internal region) AF3472a
    EB10744 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, E
Primary Accession Q9BRK5
Other Accession NP_057631.1, NP_057260.2, 51150, 20318 (mouse), 155173 (rat)
Reactivity Human
Predicted Mouse, Rat, Dog, Cow
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 41807 Da
Additional Information
Gene ID 51150
Other Names 45 kDa calcium-binding protein, Cab45, Stromal cell-derived factor 4, SDF-4, SDF4, CAB45
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSDF4 (aa161-175) Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SDF4
Synonyms CAB45
Function May regulate calcium-dependent activities in the endoplasmic reticulum lumen or post-ER compartment.
Cellular Location [Isoform 1]: Golgi apparatus lumen {ECO:0000250|UniProtKB:Q61112}
Tissue Location Ubiquitous. Isoform 5 is expressed in pancreas.
Research Areas
Citations (0)
citation

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Background

This antibody is expected to recognize both reported isoforms (NP_057631.1; NP_057260.2).

References

Transcript analyses of stromal cell derived factors (SDFs): SDF-2, SDF-4 and SDF-5 reveal a different pattern of expression and prognostic association in human breast cancer. Kang H, Escudero-Esparza A, Douglas-Jones A, Mansel RE, Jiang WG, International journal of oncology 2009 Jul 35 (1): 205-11. PMID: 19513569

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$ 341.00
Cat# AF3472a
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Availability: 7-10 days
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