Nprl3 (mouse) Antibody (internal region, N-Term)
Peptide-affinity purified goat antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Primary Accession | Q12980 |
Other Accession | NP_853547.1, 8131, 17168 (mouse), 360505 (rat) |
Reactivity | Human |
Predicted | Mouse, Rat |
Host | Goat |
Clonality | Polyclonal |
Concentration | 0.5 mg/ml |
Isotype | IgG |
Calculated MW | 63605 Da |
Gene ID | 8131 |
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Other Names | Nitrogen permease regulator 3-like protein, -14 gene protein, Alpha-globin regulatory element-containing gene protein, Protein CGTHBA, NPRL3, C16orf35, CGTHBA, MARE |
Format | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | Nprl3 (mouse) Antibody (internal region, N-Term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | NPRL3 {ECO:0000303|PubMed:26505888, ECO:0000312|HGNC:HGNC:14124} |
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Function | As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the mTORC1 pathway (PubMed:23723238, PubMed:29590090, PubMed:35338845). In response to amino acid depletion, the GATOR1 complex has GTPase activating protein (GAP) activity and strongly increases GTP hydrolysis by RagA/RRAGA (or RagB/RRAGB) within heterodimeric Rag complexes, thereby turning them into their inactive GDP-bound form, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling (PubMed:23723238, PubMed:29590090, PubMed:35338845). In the presence of abundant amino acids, the GATOR1 complex is negatively regulated by GATOR2, the other GATOR subcomplex, in this amino acid-sensing branch of the TORC1 pathway (PubMed:23723238). |
Cellular Location | Lysosome membrane. Note=Localization to lysosomes is mediated by the KICSTOR complex and is amino acid-independent. |
Tissue Location | Widely expressed. Expressed in the frontal lobe cortex as well as in the temporal, parietal, and occipital lobes (PubMed:27173016, PubMed:26505888). |
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Provided below are standard protocols that you may find useful for product applications.
References
Conservation of position and
sequence of a novel, widely expressed gene containing the major human
alpha-globin regulatory element. Vyas P, Vickers MA, Picketts DJ, Higgs DR. Genomics. 1995 Oct 10;29(3):679-89. PMID: 8575760
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