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KD-Validated Anti-ACAP2 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-ACAP2 Rabbit Monoclonal Antibody AGI1005
    Western blotting analysis using anti-ACAP2 antibody (Cat#61116). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ACAP2 antibody (Cat#61116, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). ACAP2, ArfGAP with coiled-coil, ankyrin repeat and PH domains 2.
    detail
  • WB - KD-Validated Anti-ACAP2 Rabbit Monoclonal Antibody AGI1005
    Western blotting analysis using anti-ACAP2 antibody (Cat#61116). ACAP2 expression in wild type (WT) and ACAP2 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-ACAP2 antibody (Cat#61116, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • FC - KD-Validated Anti-ACAP2 Rabbit Monoclonal Antibody AGI1005
    Flow cytometric analysis of ACAP2 expression in HT-1080 cells using ACAP2 antibody (Cat#61116, 1:2,000). Green, isotype control; red, ACAP2.
    detail
  • ICC - KD-Validated Anti-ACAP2 Rabbit Monoclonal Antibody AGI1005
    Immunocytochemical staining of HT-1080 cells with ACAP2 antibody (Cat#61116, 1:1,000). Nuclei were stained blue with DAPI; ACAP2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession Q15057
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB1565
Calculated MW Predicted, 88 kDa , observed, 88 kDa
Gene Name ACAP2
Aliases ACAP2; ArfGAP With Coiled-Coil, Ankyrin Repeat And PH Domains 2; KIAA0041; CNT-B2; CENTB2; Arf-GAP With Coiled-Coil, ANK Repeat And PH Domain-Containing Protein 2; Centaurin-Beta-2; Arf GAP With Coiled Coil, ANK Repeat And PH Domains 2; Centaurin, Beta 2; Cnt-B2
Immunogen A synthesized peptide derived from human ACAP2
Additional Information
Gene ID 23527
Other Names Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2, Centaurin-beta-2, Cnt-b2, ACAP2, CENTB2, KIAA0041
Protein Information
Name ACAP2
Synonyms CENTB2, KIAA0041
Function GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6). Doesn't show GAP activity for RAB35 (PubMed:30905672).
Cellular Location Cell membrane. Endosome membrane; Peripheral membrane protein. Note=Colocalizes with RAB35 at the membrane protrusions of HEK293T cells.
Tissue Location Widely expressed. Highest level in lung.
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1005
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