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KD-Validated Anti-Rab9 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Rab9 Rabbit Monoclonal Antibody AGI1007
    Western blotting analysis using anti-Rab9 antibody (Cat#AGI1007). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Rab9 antibody (Cat#AGI1007, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-Rab9 Rabbit Monoclonal Antibody AGI1007
    Western blotting analysis using anti-Rab9 antibody (Cat#AGI1007). Rab9 expression in wild type (WT) and Rab9 shRNA knockdown (KD) Hela cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Rab9 antibody (Cat#AGI1007, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-Rab9 Rabbit Monoclonal Antibody AGI1007
    Flow cytometric analysis of Rab9 expression in HepG2 cells using Rab9 antibody (Cat#AGI1007, 1:2,000). Green, isotype control; red, Rab9.
    detail
  • ICC - KD-Validated Anti-Rab9 Rabbit Monoclonal Antibody AGI1007
    Immunocytochemical staining of HepG2 cells with Rab9 antibody (Cat#AGI1007, 1:1,000). Nuclei were stained blue with DAPI; Rab9 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession P51151
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB1940
Calculated MW Predicted, 23 kDa, observed, 23 kDa
Gene Name RAB9A
Aliases RAB9A; RAB9A, Member RAS Oncogene Family; RAB9; RAB9, Member RAS Oncogene Family; Ras-Related Protein Rab-9A
Immunogen A synthesized peptide derived from human Rab9A
Additional Information
Gene ID 9367
Other Names Ras-related protein Rab-9A, 3.6.5.2, RAB9A (HGNC:9792), RAB9
Protein Information
Name RAB9A (HGNC:9792)
Synonyms RAB9
Function The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (By similarity). RAB9A is involved in the transport of proteins between the endosomes and the trans-Golgi network (TGN) (PubMed:34793709). Specifically uses NDE1/NDEL1 as an effector to interact with the dynein motor complex in order to control retrograde trafficking of RAB9-associated late endosomes to the TGN (PubMed:34793709). Involved in the recruitment of SGSM2 to melanosomes and is required for the proper trafficking of melanogenic enzymes TYR, TYRP1 and DCT/TYRP2 to melanosomes in melanocytes (By similarity).
Cellular Location Cell membrane; Lipid-anchor; Cytoplasmic side. Endoplasmic reticulum membrane. Golgi apparatus membrane. Late endosome. Cytoplasmic vesicle, phagosome membrane; Lipid-anchor; Cytoplasmic side. Cytoplasmic vesicle, phagosome. Cytoplasmic vesicle membrane. Melanosome {ECO:0000250|UniProtKB:Q9R0M6}. Note=Colocalizes with OSBPL1A at the late endosome (PubMed:16176980). Recruited to phagosomes containing S.aureus or M.tuberculosis (PubMed:21255211). Mainly localizes to late endosomes and partially localizes to Golgi (PubMed:34793709) Colocalizes with NDE1 to membrane vesicles (PubMed:34793709)
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$ 149.00
$ 499.00
Cat# AGI1007
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