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KD-Validated Anti-Nucleoporin 155 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Nucleoporin 155 Rabbit Monoclonal Antibody AGI1020
    Western blotting analysis using anti-Nucleoporin 155 antibody (Cat#61137). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Nucleoporin 155 antibody (Cat#61137, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-Nucleoporin 155 Rabbit Monoclonal Antibody AGI1020
    Western blotting analysis using anti-Nucleoporin 155 antibody (Cat#61137). Nucleoporin 155 expression in wild type (WT) and nucleoporin 155 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Nucleoporin 155 antibody (Cat#61137, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • FC - KD-Validated Anti-Nucleoporin 155 Rabbit Monoclonal Antibody AGI1020
    Flow cytometric analysis of Nucleoporin 155 expression in HepG2 cells using Nucleoporin 155 antibody (Cat#61137, 1:2,000). Green, isotype control; red, Nucleoporin 155.
    detail
  • ICC - KD-Validated Anti-Nucleoporin 155 Rabbit Monoclonal Antibody AGI1020
    Immunocytochemical staining of HepG2 cells with Nucleoporin 155 antibody (Cat#61137, 1:1,000). Nuclei were stained blue with DAPI; Nucleoporin 155 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession O75694
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB 2010
Calculated MW Predicted, 155 kDa , observed, 155 kDa
Gene Name NUP155
Aliases NUP155; Nucleoporin 155; KIAA0791; N155; Nuclear Pore Complex Protein Nup155; 155 KDa Nucleoporin; Nucleoporin 155kDa; Nucleoporin Nup155; Nucleoporin 155kD; ATFB15
Immunogen A synthesized peptide derived from human NUP155
Additional Information
Gene ID 9631
Other Names Nuclear pore complex protein Nup155, 155 kDa nucleoporin, Nucleoporin Nup155, NUP155, KIAA0791
Protein Information
Name NUP155
Synonyms KIAA0791
Function Essential component of nuclear pore complex. Could be essessential for embryogenesis. Nucleoporins may be involved both in binding and translocating proteins during nucleocytoplasmic transport.
Cellular Location Nucleus, nuclear pore complex {ECO:0000250|UniProtKB:P37199}. Nucleus membrane {ECO:0000250|UniProtKB:P37199}; Peripheral membrane protein {ECO:0000250|UniProtKB:P37199}; Cytoplasmic side {ECO:0000250|UniProtKB:P37199}. Nucleus membrane {ECO:0000250|UniProtKB:P37199}; Peripheral membrane protein {ECO:0000250|UniProtKB:P37199}; Nucleoplasmic side {ECO:0000250|UniProtKB:P37199}. Note=In mitosis, assumes a diffuse cytoplasmic distribution probably as a monomer, before reversing back into a punctate nuclear surface localization at the end of mitosis {ECO:0000250|UniProtKB:P37199}
Tissue Location Expressed in all tissues tested, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas
Research Areas
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$ 149.00
$ 499.00
Cat# AGI1020
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