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KD-Validated Anti-Histone deacetylase 9 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Histone deacetylase 9 Rabbit Monoclonal Antibody AGI1037
    Western blotting analysis using anti-Histone deacetylase 9 antibody (Cat#61155). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Histone deacetylase 9 antibody (Cat#61155, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-Histone deacetylase 9 Rabbit Monoclonal Antibody AGI1037
    Western blotting analysis using anti-Histone deacetylase 9 antibody (Cat#61155). Histone deacetylase 9 expression in wild type (WT) and histone deacetylase 9 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Histone deacetylase 9 antibody (Cat#61155, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • FC - KD-Validated Anti-Histone deacetylase 9 Rabbit Monoclonal Antibody AGI1037
    Flow cytometric analysis of Histone deacetylase 9 expression in HepG2 cells using Histone deacetylase 9 antibody (Cat#61155, 1:2,000). Green, isotype control; red, Histone deacetylase 9.
    detail
  • ICC - KD-Validated Anti-Histone deacetylase 9 Rabbit Monoclonal Antibody AGI1037
    Immunocytochemical staining of HepG2 cells with Histone deacetylase 9 antibody (Cat#61155, 1:1,000). Nuclei were stained blue with DAPI; Histone deacetylase 9 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession Q9UKV0
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB 2045
Calculated MW Predicted, 111 kDa , observed, 160 kDa
Gene Name HDAC9
Aliases HDAC9; Histone Deacetylase 9; HDAC7B; MITR; HD7; KIAA0744; HDAC; Histone Deacetylase 7B; EC 3.5.1.98; HDAC; HD7b; HDRP; HD9; MEF-2 Interacting Transcription Repressor (MITR) Protein; MEF2-Interacting Transcription Repressor MITR; Histone Deacetylase 4/5-Related Protein; Histone Deacetylase-Related Protein; HDAC9FL; ARCND4; HDAC9B
Immunogen A synthesized peptide derived from human HDAC9
Additional Information
Gene ID 9734
Other Names Histone deacetylase 9, HD9, 3.5.1.98, Histone deacetylase 7B, HD7, HD7b, Histone deacetylase-related protein, MEF2-interacting transcription repressor MITR, HDAC9, HDAC7, HDAC7B, HDRP, KIAA0744, MITR
Protein Information
Name HDAC9
Synonyms HDAC7, HDAC7B, HDRP, KIAA0744, MITR
Function Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Represses MEF2-dependent transcription.
Cellular Location Nucleus.
Tissue Location Broadly expressed, with highest levels in brain, heart, muscle and testis. Isoform 3 is present in human bladder carcinoma cells (at protein level).
Research Areas
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$ 149.00
$ 499.00
Cat# AGI1037
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