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KD-Validated Anti-Prohibitin Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Prohibitin Rabbit Monoclonal Antibody AGI1052
    Western blotting analysis using anti-prohibitin antibody (Cat#61170). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-prohibitin antibody (Cat#61170, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-Prohibitin Rabbit Monoclonal Antibody AGI1052
    Western blotting analysis using anti-prohibitin antibody (Cat#61170). Prohibitin expression in wild type (WT) and prohibitin (PHB1) shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-prohibitin antibody (Cat#61170, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • FC - KD-Validated Anti-Prohibitin Rabbit Monoclonal Antibody AGI1052
    Flow cytometric analysis of prohibitin expression in HT-1080 cells using prohibitin antibody (Cat#61170, 1:2,000). Green, isotype control; red, prohibitin.
    detail
  • ICC - KD-Validated Anti-Prohibitin Rabbit Monoclonal Antibody AGI1052
    Immunocytochemical staining of HT-1080 cells with prohibitin antibody (Cat#61170, 1:1,000). Nuclei were stained blue with DAPI; Prohibitin was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession P35232
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 24GB6045
Calculated MW Predicted, 30 kDa, observed, 30 kDa
Gene Name PHB1
Aliases Prohibitin 1; PHB; Prohibitin; Epididymis Secretory Sperm Binding Protein Li 54e; Epididymis Luminal Protein 215; HEL-S-54e; HEL-215
Immunogen A synthesized peptide derived from human Prohibitin
Additional Information
Gene ID 5245
Other Names Prohibitin 1, PHB1 {ECO:0000303|PubMed:28017329, ECO:0000312|HGNC:HGNC:8912}
Protein Information
Name PHB1 {ECO:0000303|PubMed:28017329, ECO:0000312|HGNC:HGNC:8912}
Function Protein with pleiotropic attributes mediated in a cell- compartment- and tissue-specific manner, which include the plasma membrane-associated cell signaling functions, mitochondrial chaperone, and transcriptional co-regulator of transcription factors in the nucleus (PubMed:11302691, PubMed:20959514, PubMed:28017329, PubMed:31522117). Plays a role in adipose tissue and glucose homeostasis in a sex-specific manner (By similarity). Contributes to pulmonary vascular remodeling by accelerating proliferation of pulmonary arterial smooth muscle cells (By similarity).
Cellular Location Mitochondrion inner membrane. Nucleus. Cytoplasm. Cell membrane
Tissue Location Widely expressed in different tissues.
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1052
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