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KD-Validated Anti-NDUFS3 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-NDUFS3 Rabbit Monoclonal Antibody AGI1071
    Western blotting analysis using anti-NDUFS3 antibody (Cat#61193). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NDUFS3 antibody (Cat#61193, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-NDUFS3 Rabbit Monoclonal Antibody AGI1071
    Western blotting analysis using anti-NDUFS3 antibody (Cat#61193). NDUFS3 expression in wild type (WT) and NDUFS3 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-NDUFS3 antibody (Cat#61193, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • FC - KD-Validated Anti-NDUFS3 Rabbit Monoclonal Antibody AGI1071
    Flow cytometric analysis of NDUFS3 expression in HepG2 cells using NDUFS3 antibody (Cat#61193, 1:2,000). Green, isotype control; red, NDUFS3.
    detail
  • ICC - KD-Validated Anti-NDUFS3 Rabbit Monoclonal Antibody AGI1071
    Immunocytochemical staining of HepG2 cells with NDUFS3 antibody (Cat#61193, 1:1,000). Nuclei were stained blue with DAPI; NDUFS3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession O75489
Reactivity Human
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB 2145
Calculated MW Predicted, 30 kDa, observed, 26 kDa
Gene Name NDUFS3
Aliases NDUFS3; NADH:Ubiquinone Oxidoreductase Core Subunit S3; NADH Dehydrogenase [Ubiquinone] Iron-Sulfur Protein 3, Mitochondrial; CI-30; NADH Dehydrogenase (Ubiquinone) Fe-S Protein 3, 30kDa (NADH-Coenzyme Q Reductase); NADH-Ubiquinone Oxidoreductase 30 KDa Subunit; Complex I 30kDa Subunit; Complex I-30kD; CI-30kD; NADH Dehydrogenase (Ubiquinone) Fe-S Protein 3 (30kD) (NADH-Coenzyme Q Reductase); NADH Dehydrogenase-Ubiquinone 30 KDa Subunit; EC 1.6.99.5; EC 7.1.1.2; MC1DN8
Immunogen A synthesized peptide derived from human NDUFS3
Additional Information
Gene ID 4722
Other Names NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial, 7.1.1.2, Complex I-30kD, CI-30kD, NADH-ubiquinone oxidoreductase 30 kDa subunit, NDUFS3
Protein Information
Name NDUFS3
Function Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor (PubMed:14729820, PubMed:30140060). Essential for the catalytic activity and assembly of complex I (PubMed:14729820, PubMed:24028823, PubMed:30140060).
Cellular Location Mitochondrion inner membrane; Peripheral membrane protein; Matrix side
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1071
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