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KD-Validated Anti-Carbamoyl-phosphate synthase 1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Carbamoyl-phosphate synthase 1 Rabbit Monoclonal Antibody AGI1107
    Western blotting analysis using anti-Carbamoyl-phosphate synthase 1 antibody (Cat#AGI1107). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Carbamoyl-phosphate synthase 1 antibody (Cat#AGI1107, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-Carbamoyl-phosphate synthase 1 Rabbit Monoclonal Antibody AGI1107
    Western blotting analysis using anti-Carbamoyl-phosphate synthase 1 antibody (Cat#AGI1107). Carbamoyl-phosphate synthase 1 expression in wild type (WT) and carbamoyl-phosphate synthase 1 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Carbamoyl-phosphate synthase 1 antibody (Cat#AGI1107, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • ICC - KD-Validated Anti-Carbamoyl-phosphate synthase 1 Rabbit Monoclonal Antibody AGI1107
    Immunocytochemical staining of HepG2 cells with Carbamoyl-phosphate synthase 1 antibody (Cat#AGI1107, 1:1,000). Nuclei were stained blue with DAPI; Carbamoyl-phosphate synthase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, ICC
Primary Accession P31327
Reactivity Human
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB 2365
Calculated MW Predicted, 165 kDa , observed, 150 kDa
Gene Name CPS1
Aliases CPS1; Carbamoyl-Phosphate Synthase 1; GATD6; Carbamoyl-Phosphate Synthase [Ammonia], Mitochondrial; Carbamoyl-Phosphate Synthase 1, Mitochondrial; Carbamoyl-Phosphate Synthase (Ammonia); EC 6.3.4.16; Carbamoyl-Phosphate Synthetase 1, Mitochondrial; Carbamoyl-Phosphate Synthetase I; Carbamoylphosphate Synthetase I; CPSase I; CPSASE1; PHN
Immunogen A synthesized peptide derived from human CPS1
Additional Information
Gene ID 1373
Other Names Carbamoyl-phosphate synthase [ammonia], mitochondrial, 6.3.4.16, Carbamoyl-phosphate synthetase I, CPSase I, CPS1
Protein Information
Name CPS1
Function Involved in the urea cycle of ureotelic animals where the enzyme plays an important role in removing excess ammonia from the cell.
Cellular Location Mitochondrion. Nucleus, nucleolus. Cell membrane {ECO:0000250|UniProtKB:Q8C196}; Peripheral membrane protein; Extracellular side {ECO:0000250|UniProtKB:Q8C196} Note=Localizes to the cell surface of hepatocytes {ECO:0000250|UniProtKB:Q8C196}
Tissue Location Primarily in the liver and small intestine.
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1107
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