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KD-Validated Anti-Bridging integrator 1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Bridging integrator 1 Rabbit Monoclonal Antibody AGI1166
    Western blotting analysis using anti-Bridging integrator 1 antibody (Cat#61353). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Bridging integrator 1 antibody (Cat#61353, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-Bridging integrator 1 Rabbit Monoclonal Antibody AGI1166
    Western blotting analysis using anti-Bridging integrator 1 antibody (Cat#61353). Bridging integrator 1 expression in wild type (WT) and Bridging integrator 1 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Bridging integrator 1 antibody (Cat#61353, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • FC - KD-Validated Anti-Bridging integrator 1 Rabbit Monoclonal Antibody AGI1166
    Flow cytometric analysis of Bridging integrator 1 expression in C2C12 cells using Bridging integrator 1 antibody (Cat#61353, 1:2,000). Green, isotype control; red, Bridging integrator 1.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC
Primary Accession O00499
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB3010
Calculated MW Predicted, 65 kDa , observed, 50, 60, 65 kDa
Gene Name BIN1
Aliases BIN1; Bridging Integrator 1; Myc Box-Dependent-Interacting Protein 1; Amphiphysin II; SH3P9; AMPH2; AMPHL; Box-Dependent Myc-Interacting Protein 1; Amphiphysin-Like Protein; Amphiphysin 2; Box Dependant MYC Interacting Protein 1; CNM2
Immunogen A synthesized peptide derived from human BIN1
Additional Information
Gene ID 274
Other Names Myc box-dependent-interacting protein 1, Amphiphysin II, Amphiphysin-like protein, Box-dependent myc-interacting protein 1, Bridging integrator 1, BIN1, AMPHL
Protein Information
Name BIN1
Synonyms AMPHL
Function Is a key player in the control of plasma membrane curvature, membrane shaping and membrane remodeling. Required in muscle cells for the formation of T-tubules, tubular invaginations of the plasma membrane that function in depolarization-contraction coupling (PubMed:24755653). Is a negative regulator of endocytosis (By similarity). Is also involved in the regulation of intracellular vesicles sorting, modulation of BACE1 trafficking and the control of amyloid-beta production (PubMed:27179792). In neuronal circuits, endocytosis regulation may influence the internalization of PHF-tau aggregates (By similarity). May be involved in the regulation of MYC activity and the control cell proliferation (PubMed:8782822). Has actin bundling activity and stabilizes actin filaments against depolymerization in vitro (PubMed:28893863).
Cellular Location [Isoform BIN1]: Nucleus. Cytoplasm Endosome {ECO:0000250|UniProtKB:O08539}. Cell membrane, sarcolemma, T- tubule {ECO:0000250|UniProtKB:O08839}
Tissue Location Ubiquitous. Highest expression in the brain and muscle (PubMed:9182667). Expressed in oligodendrocytes (PubMed:27488240). Isoform IIA is expressed only in the brain, where it is detected in the gray matter, but not in the white matter (PubMed:27488240). Isoform BIN1 is widely expressed with highest expression in skeletal muscle.
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$ 149.00
$ 499.00
Cat# AGI1166
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