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KD-Validated Anti-ALAS1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-ALAS1 Rabbit Monoclonal Antibody AGI1174
    Western blotting analysis using anti-ALAS1 antibody (Cat#AGI1174). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ALAS1 antibody (Cat#AGI1174, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-ALAS1 Rabbit Monoclonal Antibody AGI1174
    Western blotting analysis using anti-ALAS1 antibody (Cat#AGI1174). ALAS1 expression in wild type (WT) and ALAS1 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-ALAS1 antibody (Cat#AGI1174, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P13196
Reactivity Rat, Human
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB3190
Calculated MW Predicted, 71 kDa , observed, 71 kDa
Gene Name ALAS1
Aliases ALAS1; 5'-Aminolevulinate Synthase 1; ALAS-H; ALAS3; 5-Aminolevulinate Synthase, Non-Specific, Mitochondrial; Aminolevulinate, Delta-, Synthase 1; 5-Aminolevulinic Acid Synthase 1; Delta-Aminolevulinate Synthase 1; Delta-ALA Synthase 1; EC 2.3.1.37; ALASH; ALAS; 5-Aminolevulinate Synthase, Nonspecific, Mitochondrial; Migration-Inducing Protein 4; EC 2.3.1; MIG4
Immunogen A synthesized peptide derived from human Alas1
Additional Information
Gene ID 211
Other Names 5-aminolevulinate synthase, non-specific, mitochondrial, ALAS-H, 2.3.1.37, 5-aminolevulinic acid synthase 1, Delta-ALA synthase 1, Delta-aminolevulinate synthase 1, ALAS1, ALAS3, ALASH
Protein Information
Name ALAS1
Synonyms ALAS3, ALASH
Function Catalyzes the pyridoxal 5'-phosphate (PLP)-dependent condensation of succinyl-CoA and glycine to form aminolevulinic acid (ALA), with CoA and CO2 as by-products.
Cellular Location Mitochondrion inner membrane {ECO:0000250|UniProtKB:P22557}; Peripheral membrane protein {ECO:0000250|UniProtKB:P22557}. Note=Localizes to the matrix side of the mitochondrion inner membrane. {ECO:0000250|UniProtKB:P22557}
Research Areas
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$ 149.00
$ 499.00
Cat# AGI1174
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