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KD-Validated Anti-CDC23 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-CDC23 Rabbit Monoclonal Antibody AGI1187
    Western blotting analysis using anti-CDC23 antibody (Cat#AGI1187). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CDC23 antibody (Cat#AGI1187, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-CDC23 Rabbit Monoclonal Antibody AGI1187
    Western blotting analysis using anti-CDC23 antibody (Cat#AGI1187). CDC23 expression in wild type (WT) and CDC23 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-CDC23 antibody (Cat#AGI1187, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • ICC - KD-Validated Anti-CDC23 Rabbit Monoclonal Antibody AGI1187
    Immunocytochemical staining of C2C12 cells with CDC23 antibody (Cat#AGI1187, 1:1,000). Nuclei were stained blue with DAPI; CDC23 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, ICC
Primary Accession Q9UJX2
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB3420
Calculated MW Predicted, 69 kDa , observed, 64 kDa
Gene Name CDC23
Aliases CDC23; Cell Division Cycle 23; ANAPC8; APC8; CUT23; Cell Division Cycle Protein 23 Homolog; Anaphase-Promoting Complex Subunit 8; Cyclosome Subunit 8; CDC23 (Cell Division Cycle 23, Yeast, Homolog); Cell Division Cycle 23 Homolog (S. Cerevisiae); Anaphase Promoting Complex Subunit 8; Cell Division Cycle 23 Homolog
Immunogen A synthesized peptide derived from human Cdc23/APC8
Additional Information
Gene ID 8697
Other Names Cell division cycle protein 23 homolog, Anaphase-promoting complex subunit 8, APC8, Cyclosome subunit 8, CDC23, ANAPC8
Protein Information
Name CDC23
Synonyms ANAPC8
Function Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle (PubMed:18485873). The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (PubMed:18485873). The APC/C complex catalyzes assembly of branched 'Lys-11'-/'Lys-48'-linked branched ubiquitin chains on target proteins (PubMed:29033132).
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$ 149.00
$ 499.00
Cat# AGI1187
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