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KD-Validated Anti-Calmegin Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Calmegin Rabbit Monoclonal Antibody AGI1200
    Western blotting analysis using anti-Calmegin antibody (Cat#AGI1200). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Calmegin antibody (Cat#AGI1200, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-Calmegin Rabbit Monoclonal Antibody AGI1200
    Western blotting analysis using anti-Calmegin antibody (Cat#AGI1200). Calmegin expression in wild type (WT) and Calmegin shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Calmegin antibody (Cat#AGI1200, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • ICC - KD-Validated Anti-Calmegin Rabbit Monoclonal Antibody AGI1200
    Immunocytochemical staining of HepG2 cells with Calmegin antibody (Cat#AGI1200, 1:1,000). Nuclei were stained blue with DAPI; Calmegin was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, ICC
Primary Accession O14967
Reactivity Human
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB3280
Calculated MW Predicted, 70 kDa, observed, 80 kDa
Gene Name CLGN
Aliases CLGN; Calmegin; Testis Tissue Sperm-Binding Protein Li 79P
Immunogen A synthesized peptide derived from human Calmegin
Additional Information
Gene ID 1047
Other Names Calmegin, CLGN
Protein Information
Name CLGN
Function Functions during spermatogenesis as a chaperone for a range of client proteins that are important for sperm adhesion onto the egg zona pellucida and for subsequent penetration of the zona pellucida. Required for normal sperm migration from the uterus into the oviduct. Required for normal male fertility. Binds calcium ions (By similarity).
Cellular Location Endoplasmic reticulum membrane; Single-pass type I membrane protein
Tissue Location Detected in testis (at protein level). Detected in testis.
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1200
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